Words similar to phosphotyrosine
Example sentences for: phosphotyrosine
How can you use “phosphotyrosine” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine if the constitutive Syk activity observed in unstimulated cells carrying the (K395A/3F) transgene was accompanied by constitutive Syk tyrosine phosphorylation, the levels of phosphotyrosine on Syk were determined by immunoblotting after immunoprecipitation (Figure 3F).
IgM - immunoglobulin of the M isotype; ova - chicken ovalbumin; PY - phosphotyrosine; PLCγ2 - phospholipase C γ2; anti - antibody or antiserum generated against indicated antigen; RT-PCR - reverse transcriptase polymerase chain reaction; ICKA - immune-complex kinase assay; ITAM - immunoreceptor tyrosine-based activation motifs; SH2 and SH3 - Src homology domains 2 and 3; PBS - phosphate-buffered saline.
Further analysis demonstrated neither increases in phosphotyrosine nor phosphorylation of PLCγ2.
However, even when more protein was present in the immunoprecipitate, little if any phosphotyrosine was detected in unstimulated cells (lanes 13 and 15).
In addition, phosphotyrosine residues in Syk would provide additional sites for protein-protein interactions.