Example sentences for: phosphotransferase

How can you use “phosphotransferase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • These include the SBHM of the phosphotransferase system enzyme II (glucose permease or PTS-EII) found in several lineages of free-living bacteria, the one at the C-termini of cytochrome F from cyanobacteria and chloroplasts, and the NlpD-like cell wall peptidases that are present in most bacteria.

  • A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].

  • This approach is also appealing because it relies on reconstitution of active neomycin phosphotransferase activity, and so correctly targeted ES cell clones can be simply selected by growth in G418.

  • In contrast, we predicted that homologous recombination between the truncated Neo sequences in the targeting vector and Neo sequences within Hnf3αΔpgk-Neo ES cells would reconstitute expression of active neomycin phosphotransferase and so confer resistance to G418.

  • To test this we generated a targeting vector to replace exon 1 of the mouse hprt gene with a neomycin phosphotransferase (neo) gene that conferred resistance to te pharmacological inhibitor G418 in ES cells (fig 1a).


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