Words similar to phosphotransferase
Example sentences for: phosphotransferase
How can you use “phosphotransferase” in a sentence? Here are some example sentences to help you improve your vocabulary:
This approach is also appealing because it relies on reconstitution of active neomycin phosphotransferase activity, and so correctly targeted ES cell clones can be simply selected by growth in G418.
A Xho1/HindIII (blunt) cassette containing the Tn5 neomycin phosphotransferase ( Neo )gene, that could confer resistance to G418 and whose expression in mammalian cells was directed by a phosphoglycerate kinase-1 ( pgk) promoter, was introduced into an XbaI (blunt) site between the Hnf3α genomic sequences [ 16, 25].
dNTP, deoxyribonucleoside triphosphate; hnRNP heterogeneous nuclear ribonuclear protein; nt, nucleotide; LTR, long terminal repeat; MEF, mouse embryonic fibroblast; NPT, neomycin phosphotransferase; RT PCR, reverse transcriptase polymerase chain reaction.
To test this we generated a targeting vector to replace exon 1 of the mouse hprt gene with a neomycin phosphotransferase (neo) gene that conferred resistance to te pharmacological inhibitor G418 in ES cells (fig 1a).
The 3' splice site is downstream of the initiation codon for neomycin phosphotransferase.