Example sentences for: phosphosubstrate

How can you use “phosphosubstrate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 32P-labeled substrates were prepared and assays performed as described previously [ 18 ] . Briefly, protein phosphatase reactions contained 2 or 4 nM enzyme in a final volume of 30 μl containing buffer A (50 mM Tris, pH 7.6, 1 mM EDTA, 1 mM EGTA, 0.1% 2-mercaptoethanol), 0.4% ethanol and 10 μM radiolabeled phosphosubstrate in the absence or presence of AA [ 19 ] . Reactions were initiated by adding substrate and lipid, and incubated at 30°C for 10 to 15 min.

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