Words similar to phosphoprotein
Example sentences for: phosphoprotein
How can you use “phosphoprotein” in a sentence? Here are some example sentences to help you improve your vocabulary:
Assays, 80 μl total volume, containing 50 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 1 mM EDTA, 100 μM oleic acid (assay buffer) and [ 32P]phosphoprotein (1-2 μM PO 4 ), were conducted at 37°C as described previously [ 34] using PP5 purified from bovine brain.
Objects in nuclei recognized by antibodies specific for phosphoprotein epitopes, cytoplasmic IFs, or both, have been reported in glial and neuronal cells, in situ and in vitro . The nuclear structures appear spherical or rod-like and may have a positional relationship with nuclear pores [ 1 2 3 4 ] . Morphologically, these structures appear similar to the nuclear "speckles" that are thought to be storage sites for RNA splicing factors [ 5 6 7 ] . However, while intermediate filament (IF) phosphoproteins could be components of nuclear speckles, they are immunologically distinct.
The activity of the catalytic subunit of PP1 is controlled by its interaction with a large number of regulatory subunits, many of which also target it to specific subcellular compartments [ 7 24 33 ] . The major ones include the glycogen-targeting subunits (G M , G L ) [ 34 ] , myofibrillar-targeting subunit (M 110 ) [ 34 ] , nuclear inhibitor of PP1 (NIPP-1) [ 35 ] , PP1 nuclear targeting subunit (PNUTS) [ 36 37 ] , mitosis-regulating subunit Sds22 [ 38 39 40 ] , ribosomal protein L5 [ 41 ] and small cytosolic inhibitory proteins, I-1, I-2, and DARRP-32 (Dopamine and cAMP-regulated phosphoprotein, Mr 32,000) [ 7 23 25 ] . The physiological role of many of these interactions has been revealed in recent studies.
Preparation of phosphoprotein substrates
The BRCA1 gene encodes a 220-kDa nuclear phosphoprotein that is regulated in response to DNA damaging agents [ 5, 6, 7] and in response to estrogen-induced growth [ 8, 9, 10, 11].
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