Words similar to phosphoprotein
Example sentences for: phosphoprotein
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Objects in nuclei recognized by antibodies specific for phosphoprotein epitopes, cytoplasmic IFs, or both, have been reported in glial and neuronal cells, in situ and in vitro . The nuclear structures appear spherical or rod-like and may have a positional relationship with nuclear pores [ 1 2 3 4 ] . Morphologically, these structures appear similar to the nuclear "speckles" that are thought to be storage sites for RNA splicing factors [ 5 6 7 ] . However, while intermediate filament (IF) phosphoproteins could be components of nuclear speckles, they are immunologically distinct.
Assays, 80 μl total volume, containing 50 mM Tris-HCl, pH 7.4, 0.5 mM DTT, 1 mM EDTA, 100 μM oleic acid (assay buffer) and [ 32P]phosphoprotein (1-2 μM PO 4 ), were conducted at 37°C as described previously [ 34] using PP5 purified from bovine brain.
On the basis of sequence homo logy and similarity of three-dimensional structures, phosphoprotein phosphatases (PPases) have been classified into three families designated PPP, PPM, and PTP [reviewed in [ 1 2 3 ] . The PPP and PPM families are comprised of phosphoserine- and phosphothreonine-specific enzymes whereas the PTP family consists of phosphotyrosine-specific and dual-specificity enzymes [ 4 ] . The major members of the PPP family are PP1, PP2A, and PP2B (calcineurin) class of phosphatases.
As additional controls in these experiments, we showed that inhibitory RNA against Dsh, a cytoplasmic phosphoprotein believed to mediate Wg signaling via Fz receptors, blocks about 60% of signaling produced by co-transfection with Wg and DFz2 plasmids.
ROCKs are important downstream targets of Rho (Figure 7), catalyzing the phosphorylation of effector phosphoprotein substrates [23].
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