Words similar to phospho-
Example sentences for: phospho-
How can you use “phospho-” in a sentence? Here are some example sentences to help you improve your vocabulary:
ARPE-19 cells were treated with 1.5 mM H 2 O 2 for 1 hour and processed for immunohistochemical staining using antibodies against phospho-ERK.
PD098059, an inhibitor of MEK activation, was included in all experiments, except the ERK activation experiments, to increase the specificity of the phospho-JNK antibody which recognizes the 44 and 42 kDa ERK bands as well as bands at 54 and 46 kDa.
Four micron full coronal kidney sections from each animal were adhered to lysine-coated slides and used for immunodetection of phospho-Rb (Ser780) as follows: sections were deparaffinized and hydrated by placing in xylene for 8 minutes, followed by 3 minutes each of 100%, 96%, 80%, 70%, 50%, 30% ethanol, ending with 10 minutes in water; sections then underwent antigen retrieval (Glyca Reagent, Biogenex) followed by immunodetection (Super Sensitive Detection Kit, Biogenex) of phospho-Rb (Ser780) after a 12 hour incubation with a 1:1000 dilution of rabbit anti-phospho-Rb (Ser780) antibody (Cell Signaling) or control rabbit anti-sera (Biogenex) at 4°C.
Phospho-JNK was elevated with in 5 minutes after C2 addition and remained elevated for at least 30 min.
Phospho-ERK was detected by mouse anti-phospho-ERK antibody (Santa Cruz Biotechnology, Santa Cruz, CA, used at 1:1500) and the Vectastain ABC kit (Vector Lab.
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