Words similar to phalloidin

Example sentences for: phalloidin

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Although phalloidin staining and immunolabeling of tubulin were poor in cells prepared by the DOTMAC/PFA method (Figure 3Rand 3T), this method provided the widest spectrum of reactivity with the probes investigated here (Figure 3and Table 1).
Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.
The DOTMAC/PFA method provided excellent morphological preservation of actin stress fibers and microextensions and high intensity immunolabeling of actin, while phalloidin staining was poor probably owing to partial denaturation of actin filaments.
Rhodamine phalloidin was used to stain and visualize actin structures in the cell [ 61].
Among the coagulant fixatives, acetone produced the best results with respect to preservation of microextensions, staining of actin with either phalloidin or with monoclonal β-actin antibodies, and staining of microtubules with a monoclonal anti-β-tubulin reagent.