Example sentences for: phalloidin

How can you use “phalloidin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.

  • The cells were extracted for 5 min on ice with 200 μl of 2× detergent extraction buffer (2% of Triton X-100 or DOTMAC, 50 mM Tris-HCl, 10 mM EGTA, 200 mM NaCl, 2 mM MgCl 2 , protease inhibitor cocktail [20 μg/ml aprotinin, 20 μM E-64, 200 μM leupeptin, 200 μM p-amidinophenylmethanesulfonyl fluoride], 2 μM calyculin A, 10 mM sodium pyrophosphate, 2 μM staurosporine, 20 μM phalloidin, and 20 μg/ml taxol).

  • When we examined the development of fz mutant laterals by phalloidin staining we found that lateral initiation was not as restricted to the distal part of cells as is the case in wild type (Fig.

  • [ 22] pointed out that some microextensions may be lost during fixation and washing steps, and Nobes and Hall [ 16] added glutaraldehyde to the fixative in order to stain these delicate structures with phalloidin, but glutaraldehyde had to be omitted for immunostaining.

  • Cells were stained with the above blocking buffer containing 1 μg/ml rhodamine phalloidin for 1 h, and rinsed 3× with PBS.

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