Example sentences for: phalloidin

How can you use “phalloidin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Rhodamine phalloidin was used to stain and visualize actin structures in the cell [ 61].

  • The DOTMAC/PFA method provided excellent morphological preservation of actin stress fibers and microextensions and high intensity immunolabeling of actin, while phalloidin staining was poor probably owing to partial denaturation of actin filaments.

  • The cells were extracted for 5 min on ice with 200 μl of 2× detergent extraction buffer (2% of Triton X-100 or DOTMAC, 50 mM Tris-HCl, 10 mM EGTA, 200 mM NaCl, 2 mM MgCl 2 , protease inhibitor cocktail [20 μg/ml aprotinin, 20 μM E-64, 200 μM leupeptin, 200 μM p-amidinophenylmethanesulfonyl fluoride], 2 μM calyculin A, 10 mM sodium pyrophosphate, 2 μM staurosporine, 20 μM phalloidin, and 20 μg/ml taxol).

  • Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.

  • Among the coagulant fixatives, acetone produced the best results with respect to preservation of microextensions, staining of actin with either phalloidin or with monoclonal β-actin antibodies, and staining of microtubules with a monoclonal anti-β-tubulin reagent.


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