Words similar to phalloidin
Example sentences for: phalloidin
How can you use “phalloidin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Anti-β-actin mouse mAb (A5441), anti-β-tubulin mouse mAb (T4026), anti-vinculin mouse mAb (V9131), anti-mouse IgG FITC conjugate (F2012), anti-rabbit IgG FITC conjugate (F1262), anti-rabbit IgG TRITC conjugate (T5268), and TRITC labeled phalloidin were purchased from Sigma.
Although phalloidin staining and immunolabeling of tubulin were poor in cells prepared by the DOTMAC/PFA method (Figure 3Rand 3T), this method provided the widest spectrum of reactivity with the probes investigated here (Figure 3and Table 1).
Actin staining was done as described previously [ 8 ] using Alexa 568 phalloidin (Molecular Probes).
The cells were extracted for 5 min on ice with 200 μl of 2× detergent extraction buffer (2% of Triton X-100 or DOTMAC, 50 mM Tris-HCl, 10 mM EGTA, 200 mM NaCl, 2 mM MgCl 2 , protease inhibitor cocktail [20 μg/ml aprotinin, 20 μM E-64, 200 μM leupeptin, 200 μM p-amidinophenylmethanesulfonyl fluoride], 2 μM calyculin A, 10 mM sodium pyrophosphate, 2 μM staurosporine, 20 μM phalloidin, and 20 μg/ml taxol).
Of the 7 kinase and AKAP labeling patterns shown in this paper, RIIα is the only one that strongly associates with the z-line (contrast with the phalloidin labeling shown in fig.
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