Example sentences for: pgl

How can you use “pgl” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Various deletion constructs were created by first digesting the 3 kb genomic fragment (HindIII-NcoI) with various restriction enzymes, blunted with Klenow DNA polymerase, and then cloned into pGL2Basic luciferase reporter plasmid.

  • To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.

  • The luciferase expression vector pGL3 Control (0.

  • The PCR product was digested with Hind III and ligated into the Hind III site of pGL2CAT/Luc (a gift of R.E.


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