Words similar to pgl
Example sentences for: pgl
How can you use “pgl” in a sentence? Here are some example sentences to help you improve your vocabulary:
Various deletion constructs were created by first digesting the 3 kb genomic fragment (HindIII-NcoI) with various restriction enzymes, blunted with Klenow DNA polymerase, and then cloned into pGL2Basic luciferase reporter plasmid.
To test this, a 472 nucleotide fragment, representing the full-length human p27 Kip15'-UTR derived from transcription initiation at the major start site, was inserted into the bicistronic expression vector pGL2CAT/Luc [ 30 ] . In this vector, the first cistron, encoding chloramphenicol acetyltransferase (CAT), is proximal to the mRNA's cap structure and therefore expected to be translated by the conventional cap-dependent translation mode.
The luciferase expression vector pGL3 Control (0.
The PCR product was digested with Hind III and ligated into the Hind III site of pGL2CAT/Luc (a gift of R.E.