Example sentences for: pem

How can you use “pem” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Unspecific epitopes were blocked by incubation in PEM-BAL (PEM plus 1% BSA, 0.1% Na-azide, 0.1 M L-lysine HCl) for 1h.

  • The taxol-stabilized β-tubulin (1 μM) was also incubated in PEM buffer (100 mM PIPES, 5 mM EGTA, 2 mM MgCl 2 , pH6.

  • Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.

  • Although MTSB, which is essentially similar to PHEM, PEM, or MTPB, was shown to give the best preservation of the cytoskeleton, no morphological differences were seen between these buffers and PBS(+), when the cells were extracted with DOTMAC (data not shown).

  • Cells were washed in PEM (100 mM Pipes pH6.

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