Words similar to pelleted

Example sentences for: pelleted

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In order to observe changes at the onset of apoptosis, only adherent cell populations were trypsinized, pelleted 5 minutes at 500 × g, and lysed in ice cold lysis buffer (10 mM Tris-HCl (pH 7.5), 1 mM MgCl 2 , 1 mM EGTA, 0.1 mM PMSF, 5 mM β-mercaptoethanol, 0.5% CHAPS, 10% glycerol) for 30 minutes at 4°C.
They were then pelleted and resuspended in working buffer (blocking buffer diluted 10 x in MIB) containing the primary antibody, anti-myc (1:1000) for 1 hr on ice.
Nuclei were pelleted in a microcentrifuge for 10 sec.
The TCA-precipitated proteins were pelleted by centrifugation (20,800 × g at 4°C) for 20 min and resuspended in 2X sodium dodecyl sulfate (SDS) sample buffer [ 34 ] . Protein extracts for affinity-purification were prepared by disintegration with a French press.
The cells were then grown in 12-well plates in triplicate wells, and measurement of 3H-hypoxanthine incorporation was carried out at 24 hr post-electroporation using standard procedures [ 49 ] , except that parasites were liberated with saponin, pelleted, and counts in the pellet measured following solubilization.