Example sentences for: pellet

How can you use “pellet” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.

  • The resulting protein pellet was resuspended in 10 ml of buffer A (50 mM Tris-HCl, pH 7.5, 5 mM MgCl 2, 0.1 mM EDTA, 0.5 mM DTT, 10 % glycerol and 1 mM PMSF) and dialyzed against 500 ml of buffer A overnight.

  • Briefly, the microdissected cell pellet was incubated overnight in lysis buffer (Qiagen Inc) with proteinase-K at 55°C.

  • These mixtures were then centrifuged at 3000 rpm for 30 min, after which the supernatant for each was decanted into new tubes and the pellet discarded.

  • The pellet was dried at 22-25°C for 10 min and re-suspended in 50 μl of nuclease-free H 2 O. The re-suspended solution was heated at 56°C for 5 min and centrifuged at 10,000 rpm for 2 min.


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