Words similar to pellet
Example sentences for: pellet
How can you use “pellet” in a sentence? Here are some example sentences to help you improve your vocabulary:
Following centrifugation, the cell pellet was resuspended in ice-cold 50 mM Tris HCl buffer pH 7.4 at about 10 7cell/ml, and homogenized using a polytron at setting 6 for 10 seconds.
The resulting protein pellet was resuspended in 10 ml of buffer A (50 mM Tris-HCl, pH 7.5, 5 mM MgCl 2, 0.1 mM EDTA, 0.5 mM DTT, 10 % glycerol and 1 mM PMSF) and dialyzed against 500 ml of buffer A overnight.
Briefly, the microdissected cell pellet was incubated overnight in lysis buffer (Qiagen Inc) with proteinase-K at 55°C.
These mixtures were then centrifuged at 3000 rpm for 30 min, after which the supernatant for each was decanted into new tubes and the pellet discarded.
The pellet was dried at 22-25°C for 10 min and re-suspended in 50 μl of nuclease-free H 2 O. The re-suspended solution was heated at 56°C for 5 min and centrifuged at 10,000 rpm for 2 min.