Words similar to pedf

Example sentences for: pedf

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Complex formation between PEDF and CM components was assayed by a method using ultrafiltration through membranes of 100,000 MW exclusion limit [ 14 ] . Binding reactions were performed with a given concentration of 125I-PEDF in defined or conditioned media, and incubations with gentle rotation at 4°C for 2 h.
We have shown that the binding of PEDF to receptors in retinoblastoma cells is enhanced by the presence of extracellular heparin/HS-like GAGs, which can be found in the culture medium of retinoblastoma cells.
Recombinant PEDF was purified from BHK cells containing an expression vector with human PEDF cDNA, as previously described [ 19 ] .
The primary consequence of reducing the heparin/HS and its sulfation was to minimize a binding site required for PEDF activity.
Interactions between PEDF and extracellular GAGs can also explain the complex formed by PEDF with CM even after protease treatment of the latter.