Words similar to pe-conjugated
Example sentences for: pe-conjugated
How can you use “pe-conjugated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The following monoclonal antibodies were purchased from BD Biosciences Pharmingen (San Diego, California, United States): FITC-, Cy5-, and PE-conjugated anti-CD4, -CD8, -CD3, -CD14, -CD69, -CXCR3, -CCR3, -CCR4, and -CCR5.
Aliquots of 50 μL were incubated for 20 min at room temperature with 2 μL of annexin V-FITC from BD Pharmingen (San Diego, CA) and saturating concentrations of PE-conjugated Mab.
Antigen expression on MP was analyzed by double-labeling with annexin V-FITC and PE-conjugated Mabs.
This mixture of cells, apoptotic bodies and other cell fragments was centrifuged (300 g/5 min), washed with HBSS/BSA, labeled in aliquots with saturating concentration of PE-conjugated Mab against CD105 or CD59, washed again in HBSS/BSA and analyzed by flow cytometry.
RA synovial tissue T cells were analyzed without in vitro culture or stimulation by staining synovium mononuclear cells with FITC-conjugated anti-CD4 mono-clonal antibody (Q4120; Sigma) and PE-conjugated anti-CD45RO monoclonal antibody (UCHL-1), after which individual CD4 +CD45RO +T cells were sorted in a 96-well PCR plate (Robbins Scientific, Sunnyvale, CA, USA) using the FACStar Plus(Becton Dickinson, San Jose, CA, USA).