Words similar to pc-tubulin

Example sentences for: pc-tubulin

How can you use “pc-tubulin” in a sentence? Here are some example sentences to help you improve your vocabulary:

BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
1. In developing the quantitative tubulin ELISA, either microtubule protein from pig brain containing unknown amounts of tubulin or a known amount of PC-tubulin was used as the experimental sample.
These comparisons allowed us to calibrate the amounts of β-tubulin classes I + IV, II, and III in PC-tubulin and in pig brain samples (Table 2).
Duplicate standard curves for each antibody were made with PC-tubulin (final concentration 150 μg/ml, 3% nonfat dried milk in PBS).
Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.