Words similar to pc-tubulin
Example sentences for: pc-tubulin
How can you use “pc-tubulin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Duplicate unknown tissue samples were prepared by combining them with known levels of PC-tubulin (1 : 1), then mixing them 1 : 1 with antibody in 3% nonfat dried milk in PBS.
1. In developing the quantitative tubulin ELISA, either microtubule protein from pig brain containing unknown amounts of tubulin or a known amount of PC-tubulin was used as the experimental sample.
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
These comparisons allowed us to calibrate the amounts of β-tubulin classes I + IV, II, and III in PC-tubulin and in pig brain samples (Table 2).
Isotype determinations with the experimental samples made with known PC-tubulin were, within error, the same as the expected quantities (data not shown).