Words similar to pc-tubulin
Example sentences for: pc-tubulin
How can you use “pc-tubulin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Duplicate unknown tissue samples were prepared by combining them with known levels of PC-tubulin (1 : 1), then mixing them 1 : 1 with antibody in 3% nonfat dried milk in PBS.
Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
These comparisons allowed us to calibrate the amounts of β-tubulin classes I + IV, II, and III in PC-tubulin and in pig brain samples (Table 2).
Isotype determinations with the experimental samples made with known PC-tubulin were, within error, the same as the expected quantities (data not shown).
1. In developing the quantitative tubulin ELISA, either microtubule protein from pig brain containing unknown amounts of tubulin or a known amount of PC-tubulin was used as the experimental sample.