Words similar to passaged
Example sentences for: passaged
How can you use “passaged” in a sentence? Here are some example sentences to help you improve your vocabulary:
The strain used in this study, AD169, and the Towne strains were both used to develop vaccines [ 49 ] . Differences in infectivity have been observed between 2 of the same strains of CMV and this may be attributed to the deletion or mutation of certain tropism genes upon extensive serial propagation [ 50 51 ] . Clinical isolates (wild-type CMV) can carry more than 220 genes, and some of these genes can be lost by deletion and/or rearrangement during laboratory passage [ 50 ] . The AD169 strain used in this study has been passaged many times and because of these variations observed among the CMV strains, this may partly account for the low infectivity observed in the BJAB-B1 and P3HR-1 cells.
Cells were harvested by centrifugation (800 rpm in a clinical centrifuge) and were grown and serially passaged as previously described [ 36 ] . For experiments, cells from passages four to six were used with an initial plating density of 10-20 cells/mm 2. When added, ascorbic acid and TGFβ1 (R&D Systems) were used at 50μg/ml and 10 ng/ml, respectively.
LNCaP cells, passaged and treated under similar conditions, were used as a positive control for androgen reponsiveness.
Some fibroblasts were passaged as described for other cell types [ 37 ] , seeded onto four-chamber slides, allowed to reach similar confluence, and processed for immunohistochemistry with primary antibodies to fibroblast markers.
The cell lines MCF7/WT (ADR-sensitive) and MCF7/ADR were used in this study; they were obtained from NCI [ 13] and maintained in Dulbecco's modified Eagle's medium incubated at 37°C in 5% CO 2 -95% air at high humidity, and passaged every 2-3 days.
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