Example sentences for: passaged

How can you use “passaged” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cell lines MCF7/WT (ADR-sensitive) and MCF7/ADR were used in this study; they were obtained from NCI [ 13] and maintained in Dulbecco's modified Eagle's medium incubated at 37°C in 5% CO 2 -95% air at high humidity, and passaged every 2-3 days.

  • After 4 days, the fertile males were removed from the crosses, placed in individual numbered vials, and passaged every two days until they were all dead.

  • The fibroblasts were passaged every week.

  • These cultures were passaged once and used as secondary cultures at a density of 0.3 × 106 cells/35 mm plate.

  • Cells were harvested by centrifugation (800 rpm in a clinical centrifuge) and were grown and serially passaged as previously described [ 36 ] . For experiments, cells from passages four to six were used with an initial plating density of 10-20 cells/mm 2. When added, ascorbic acid and TGFβ1 (R&D Systems) were used at 50μg/ml and 10 ng/ml, respectively.


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