Words similar to passaged

Example sentences for: passaged

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Parallel cultures were then passaged and transfected with 6His-HA-PP1α-pTEP4m.
Primary cultures were passaged when 40% confluent and maintained in GEC media thereafter.
Cells were harvested by centrifugation (800 rpm in a clinical centrifuge) and were grown and serially passaged as previously described [ 36 ] . For experiments, cells from passages four to six were used with an initial plating density of 10-20 cells/mm 2. When added, ascorbic acid and TGFβ1 (R&D Systems) were used at 50μg/ml and 10 ng/ml, respectively.
Dengue virus strains DEN1/WP and DEN2/NGC, kindly provided by Dr. Lewis Markoff, [ 43 44 ] were passaged in monkey LLC-MK2 cells at 37°C in a humidified incubator under 5% CO2, using Medium 199 plus 10% fetal bovine serum (FBS) and 50 ug of Gentamicin per ml.
All infections were performed on subconfluent cells that were passaged between 7 and 10 times after arrival from ATCC (original stock was p18).