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The strain used in this study, AD169, and the Towne strains were both used to develop vaccines [ 49 ] . Differences in infectivity have been observed between 2 of the same strains of CMV and this may be attributed to the deletion or mutation of certain tropism genes upon extensive serial propagation [ 50 51 ] . Clinical isolates (wild-type CMV) can carry more than 220 genes, and some of these genes can be lost by deletion and/or rearrangement during laboratory passage [ 50 ] . The AD169 strain used in this study has been passaged many times and because of these variations observed among the CMV strains, this may partly account for the low infectivity observed in the BJAB-B1 and P3HR-1 cells.
As European power-brokering turned into World War I, Egypt became vital to the British, being close to the enemy Ottoman heartland, and allowing quick passage through the Suez Canal to her dominions in India, the Far East, Australia, and New Zealand.
It turns out that her baby was born before the bill was ratified, but the explanation offered was that the bill's postpartum passage gave her the confidence she needed to have another baby, later.
Passage 2 fibroblasts were trypsinized, suspended in the culture medium with 10% (v/v) dimethyl sulfoxide (Sigma) and stored as frozen aliquots.
A Congressional reexamination of the regulation drafting process led to the passage of the Indian Self-Determination Act Amendments of 1994 (Pub.