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Example sentences for: pand

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To exclude this possibility, we asked whether distinct SCF Pop1pand SCF Pop2pcomplexes bear ubiquitin ligase activity in vitro . To this end, we performed in vitro ubiquitylation assays.
Individual SCF Pop1pand SCF Pop2pcomplexes bearing ubiquitin ligase activity
While these data support the existence of distinct SCF Pop1pand SCF Pop2pcomplexes, individual binding of Pop1p and Pop2p to SCF core components in the absence of their heterodimerizing F-box protein partners does not rule out the possibility that these complexes represent inactive intermediates formed during the normal assembly of functional SCF Pop1p-Pop2pcomplexes.
Since the substrates of putative SCF Pop1pand SCF Pop2pubiquitin ligases are unknown, we adopted a substrate-independent assay originally described by Lyapina et al . [ 27 ] . For this experiment, we again used the strains harboring genomically integrated Myc epitope-tagged Pop1p or Pop2p in a background deficient in the respective heterooligomerization partner ( Δpop2 pop1-13myc , Δpop1 pop2-13myc strains).
In addition, compartmentalization of homooligomeric SCF Pop1pand SCF Pop2pcomplexes may direct the ubiquitylation of unknown nuclear and cytoplasmic substrates.