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Example sentences for: padapt
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As depicted on fig 8the electroporation of 50 μg pAdapt Luc and the injection of 5.10 9iu Ad Adapt Luc induce roughly similar luciferase expression.
The plasmid pAdapt Luc, pAdpat LacZ, pAdapt hEndo and pAdapt mhAB encoding respectively for the luciferase, β-galactosidase, human endostatine and murinised human ATF-BPTI genes were constructed as described previously [ 23 ] . The different genes are under the control of the cytomegalovirus (CMV) immediate-early promotor and terminated by the simian virus (SV40) late poly(A) signal.
As a standard, to determine the amount of adenoviral genomes and cellular DNA, a plasmid containing approximately 5000 bp of the left part of the Ad5 genome (pAdapt) was mixed with cellular DNA extracted from A549 cells.
Recombinant adenovirus vectors were generated in PER.C6™ cells by homologous recombination between an adapter plasmid (pAdapt) and the E1 deleted Ad 5 DNA plasmid as described elsewhere [ 23 ] . As a result of the absence of sequence overlap between the Adapt plasmid and the Ad5 E1 sequences integrated into the genome of PER.C6, the vector stocks used in this study did not contain replicative competent adenovirus (RCA) [ 25 ] . All vectors were produced on PER.C6™ using standard procedures [ 25 ] . Infectious units (iu)/ml were determined by end point cytopathogenic effect (CPE) assay on 911 cells [ 26 ] . Viral particles were determined by HPLC [ 27 ] . The particle to infectious unit ratio was always lower than 5.
Recombinant adenovirus vectors were generated in PER.C6™ cells by homologous recombination between an Adapter plasmid (pAdapt) and the E1 deleted Ad 5 DNA plasmid as described elsewhere [ 34 ] . The expression cassette contains a CMV promoter and SV40 poly A signal.
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