Words similar to oxyburst
Example sentences for: oxyburst
How can you use “oxyburst” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although the location of activated NADPH oxidase complex in phagolysosomes has been reported, the release of either H 2 O 2 or O 2 •- in situ upon stimulation with PMA has not been directly shown in live cells [ 10 11 ] . In order to directly measure the oxidative activity in lysosomes, I use a reduced fluorescein derivative that is conjugated with BSA, OxyBURST Green H 2 HFF BSA, as shown in Figure 1. Spectrofluorimetric analysis indicates that this reduced compound is non-fluorescent, and becomes fluorescent upon HRP-catalyzed oxidation.
In order to understand the in situ enhancement of lysosomal oxidative activity by PMA, the subcellular distribution of the oxidized OxyBURST Green H 2 HFF BSA in cells was investigated.
On the other hand, acidic lysosomes strongly stained by LysoTracker Red exhibited very weak OxyBURST fluorescence (see arrowhead).
Co-localization experiments indicate that all of the OxyBURST Green H 2 HFF BSA is distributed in lysosomes but not endosomes after this labeling procedure (see below).
This clearly eliminates the possibility that there is more OxyBURST Green H 2 HFF BSA accumulating in lysosome area 2. The experiment strongly supports the notion that the oxidative activity within the lysosomal population is heterogeneous.