Words similar to oxyburst

Example sentences for: oxyburst

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In order to demonstrate this relationship in live cells, the oxidative activities and pH in lysosomes were simultaneously monitored by OxyBURST Green H 2 HFF BSA and LysoTracker Red DND-99 which is able to qualitatively indicate acidic lysosomes.
Cells were then labeled with OxyBURST Green H 2 HFF BSA, followed by chase labeling without the reduced dye but in the presence or absence of 200 nM PMA.
The pH-dependency of fluorescence of both oxidized OxyBURST Green H 2 HFF BSA and 5-(and -6)-carboxyfluorescein were thus examined by the dual excitation ratiometric measurement in vitro , as shown in Figure 8. While 5-(and -6)-carboxyfluorescein exhibits strong pH-dependency, the experiment clearly demonstrates that fluorescence of the oxidized OxyBURST Green H 2 HFF BSA is not affected by variation of the pH within a range between 4.5 to 7.5.
This clearly eliminates the possibility that there is more OxyBURST Green H 2 HFF BSA accumulating in lysosome area 2. The experiment strongly supports the notion that the oxidative activity within the lysosomal population is heterogeneous.
To study the effect of pH on the fluorescence of oxidized OxyBURST Green H 2 HFF BSA and 5-(and -6)-carboxyfluorescein, 78 μg/mL of the oxidized dye or 4.2 μM 5-(and -6)-carboxyfluorescein were prepared in MES (4-morpholineethanesulfonic acid) buffer solutions containing 5 mM NaCl, 115 mM KCl, 1.2 mM MgSO 4 and 25 mM MES (pHs from 4.5 to 7.5).