Words similar to orthovanadate

Example sentences for: orthovanadate

How can you use “orthovanadate” in a sentence? Here are some example sentences to help you improve your vocabulary:

Smooth muscle strips were weighed and total protein was extracted by glass-glass homogenization using 50 μl per milligram dry tissue sodium dodecyl sulfate (SDS)-based extraction buffer: 25 mM Tris-HCl, pH 7.4, 2% SDS, 10% glycerol, 1 mM dithiothreitol (DTT), 1 μM leupeptin, 10 mM ethylenediaminetetraacetic acid (EDTA), 1 mM sodium orthovanadate, 5 mM NaF and 1 mM phenylmethylsulfonyl fluoride (PMSF).
For preparation of protein lysates from tissue samples (levator ani muscle; rectus fascia = ~negative control; and whole placenta = low p27 positive control) 100 tissue sections (7 μm thick) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5,200 mM NaCI, 0.25% Nonidet P-40) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride (400 μl/100 mg of tissue sections).
At this time, okadaic acid (1 μM), sodium orthovanadate (1 μM) and PD098059 (10 μM) or vehicle (0.
The cells were washed with ice-cold phosphate-buffered saline and solubilized in 2 × SDS-sample buffer containing 2 mM sodium orthovanadate and 200 mM sodium fluoride.
2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).