Example sentences for: orthovanadate

How can you use “orthovanadate” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Five hundred microliters of supplemented RIPA lysis buffer (50 mM Tris-HCI pH 7.5, 150 mM NaCI, 2 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 10 ug/ml aprotinin, 10 ug/ml leupeptin, 1 mM sodium orthovanadate) were added to each dish and cells were harvested with a rubber policeman.

  • The complexes were recovered with Protein A-Sepharose (Sigma) and washed two times with ice cold NP40 wash buffer (1% NP40, PBS, 1 mM sodium orthovanadate) and analyzed by Western blotting using either anti-Rlk serum or anti-Fyn serum (1:1000).

  • 2 , 5 mM MnCl 2 , 100 μM sodium orthovanadate).

  • Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).

  • 0], 150 mM NaCl, 0.02% sodium azide, 0.1% SDS, 100 μg/ml phenylmethylsulfonyl fluoride, 1% NP-40, 0.5% sodium deoxycholate, and 10 μM sodium orthovanadate).


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