Words similar to orthovanadate
Example sentences for: orthovanadate
How can you use “orthovanadate” in a sentence? Here are some example sentences to help you improve your vocabulary:
Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).
For preparation of protein extracts from rat, human and porcine tissues for western blot analysis, 150 cryostat tissue sections (7 μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
For preparation of protein lysates from placental tissue and ovarian cancer for western blot analysis, including control Cdk2 and normal rabbit immunoglobulin immunoprecipitates (IP), 150 cryostat tissue sections (7μm) were collected into microcentrifuge tubes and lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl, 0.25% Nonidet P-40; 400 μl/100 mg of tissue sections) containing 1 mM sodium orthovanadate, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride.
To prepare cell lysates, freshly isolated trophoblast cells and early (16-24 h) and late trophoblast cultures (72-96 h) were washed twice with ice-cold PBS, lysed by adding ice-cold lysis buffer (20 mM Tris pH 7.5, 200 mM NaCl) containing 1 mM sodium orthovanadate, 0.25% Nonidet P-40, 10 mM sodium fluoride, and 1 mM phenylmethylsulfonyl fluoride, sonicated, centrifuged, and supernatants stored at -80°C until use [ 15 ] .
0], 150 mM NaCl, 0.02% sodium azide, 0.1% SDS, 100 μg/ml phenylmethylsulfonyl fluoride, 1% NP-40, 0.5% sodium deoxycholate, and 10 μM sodium orthovanadate).
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