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Example sentences for: orc

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In fact, the largest subunit of S. pombe ORC (Orp1) has been mapped by chromatin immunoprecipitation to ars3002 and also to ars2004 , which, like ars3002 , contains multiple asymmetric A+T-rich stretches [ 16, 17].
In Xenopus, biochemical analysis of replication and cell cycle events using egg extracts has helped to understand the mechanism of eukaryotic DNA replication [ 10 ] . However, two dimensional gel electrophoresis analysis of the rDNA locus showed that replication initiated at all sites tested [ 11 ] . All six human homologs of yeast and Drosophila ORC subunits have been cloned and characterized [ 12 13 ] . Other replication proteins like Cdc6, Cdt1, MCMs, Cdc45 that are essential for initiation of DNA replication have also been reported [ 12 ] . Conservation of replication factors among higher eukaryotes suggests that functionally they may play similar roles.
In S. cerevisiae , ARS elements have been implicated to be important both for ORC binding and origin function [ 6 ] . We looked for the availability of such sequences in known human origins of replication like c-myc, lamin beta 2 and Dnmt1.
S. cerevisiae origin recognition complex (ORC) binds to ARS consensus sequences in an ATP dependent fashion and this binding is essential for both origin function and activity.
Recombinant six protein Drosophila ORC (DmORC) binds to ACE region of the Drosophila chorion gene [ 9 ] . In vivo , DmORC co-localized with the amplified chorion gene locus.