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Example sentences for: opossum
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Similarly, mutation of only two serine residues in the human alpha-2A receptor resulted in a loss of desensitization [ 12 ] . Because the EESSSSE motif in the human alpha-2A receptor is a known GRK substrate, and because eliminating two potential phosphorylation sites prevents down-reguation, phosphorylation of the opossum alpha-2C receptor may be important in the mechanism of down-regulation of this receptor.
In addition to the hydroxyl amino acid residues, acidic residues may also be an important sequence element for alpha-2 adrenergic receptor phosphorylation and regulation [ 27 28 ] . The region from 300 to 313 in the opossum alpha-2C receptor, which encompasses the EESSTSE motif, is a high acidic region, with 50% (seven of fourteen) of the residues being acidic amino acids (Figure 2).
To test this hypothesis, we mutated the opossum alpha-2C serines and threonine residues to alanines and valine, respectively, and tested whether the expressed receptors would down-regulate.
We have previously shown that norepinephrine pretreatment does not alter the radioligand K d of the opossum alpha-2C adrenergic receptor [ 20 24 ] , thus making it possible to estimate the receptor density with a single concentration of radioligand.
Sixty grams is confirmed as a critical body mass for induction of estrus in juvenile female opossums as identified in our previous study [ 7 ] . Somatic growth and attainment of a threshold body mass or energy reserves are known requisites for puberty in many mammals including the tammar wallaby [ 26 ] , sheep [ 27 28 ] , and several species of rodents [ 1 ] , but these factors seldom act independently of environmental factors such as photoperiod [ 26 27 ] or social cues [ 3 29 ] . The opossum is unusual in this regard, because social cues in the form of male pheromone appear to stimulate both somatic growth and ovarian activation.
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