Words similar to opossum
Example sentences for: opossum
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Mutations of the opossum alpha-2C adrenergic receptor cDNA in pRc/CMV vector described previously [ 21 ] was accomplished using the QuickChange™ Site Directed Mutagenesis Kit (Stratagene, LaJolla, CA).
The opossum alpha-2C adrenergic receptor has a very similar acidic serine motif (EESSTSE) in the third intracellular loop (Figure 1; [ 21 ] ), whereas the human alpha-2C receptor has a DESSAAAE sequence in the same region (Figure 2; [ 22 ] ). Thus, we postulated that the lack of down-regulation in the human alpha-2C was related to having only two rather than four serines in the motif, and furthermore that the ability of the opossum alpha-2C to undergo down-regulation was dependent on having all four serine/threonine residues in the EESSTSE motif.
The opossum alpha-2C adrenergic receptor has a EESSTSE acidic serine/threonine motif in the third intracellular loop (Figures 1and 2), which could be a substrate for GRK based on the EESSSSD motif of the alpha-2A subtype.
We have previously shown that norepinephrine pretreatment does not alter the radioligand K d of the opossum alpha-2C adrenergic receptor [ 20 24 ] , thus making it possible to estimate the receptor density with a single concentration of radioligand.
Thus, we postulated that the lack of down-regulation in the human alpha-2C subtype is related to having only two rather than four serines in the motif, and that mutating the threonine and serine residues at the end of the opossum EESSTSE motif to valine and alanine residues to give EESSVAE might prevent down-regulation of the opossum alpha-2C receptor.
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