Words similar to oh-dg
Example sentences for: oh-dg
How can you use “oh-dg” in a sentence? Here are some example sentences to help you improve your vocabulary:
The reason for this is that we found high levels of background staining with the anti-8-OH-dG antibodies, which is not unusual when using streptavidin/biotin detection systems in immunohistochemistry [ 39 ] . Unfortunately, secondary antibodies directly linked to a fluorophore or a detection enzyme were not sensitive enough to detect the 8-OH-dG adduct in our hands.
Ogg1 knockout mice possess increased levels of 8-OH-dG in their genome, particularly in the mitochondrial genome, but do not seem to exhibit an increased frequency of malignancies [ 44 45 46 ] . It was postulated that the consequences of 8-OH-dG adducts and Ogg1 deficiency affects mainly cells with high oxygen metabolism and slow proliferation, such as liver cells [ 45 ] . While β-cells were not examined in that study, they certainly exhibit both high oxidative metabolism and a low rate of proliferation, consistent with them being a target for the effects of 8-OH-dG adducts.
In light of the previous publication on the role of 8-OH-dG adducts in a rat model of type 2 diabetes [ 31 ] , and the finding that the Ogg1 gene was over-expressed in human type 2 diabetes, we evaluated the expression of Ogg1 protein in pancreas specimens from type 2 diabetic and healthy subjects, as a marker for β-cell oxidative DNA damage in type 2 diabetes.
Unfortunately, we were unable to obtain specific staining with antibodies to 8-OH-dG.
We interpret the upregulation of Ogg1 as a response to an increased level of 8-OH-dG DNA, the lesion that it repairs.
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