Example sentences for: nups

How can you use “nups” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Experiments using immuno-fluorescence in vertebrate cells are beginning to order the recruitment and timing of incorporation of individual nucleoporins during post-mitotic re-assembly [ 12 13 14 15 ] . Additionally, in vitro assembly studies with Xenopus egg or mitotic cell extracts have revealed several intermediates in NPC assembly and demonstrated a requirement for an intact double nuclear membrane prior to post-mitotic NPC assembly [ 16 17 18 19 ] . The modular nature of the NPC structure has also suggested that subcomplexes of discrete Nups may initially form and provide building blocks for biogenesis.

  • To determine if additional, non-GFP tagged Nups were mislocalized in these cells, we used indirect immunofluorescence (IF) microscopy with antibodies directed against either Nup116p or Pom152p.

  • These Nups are localized throughout the NPC structure [ 1 ] and indicate a global defect in NPC assembly/structure.

  • The lack of secretion defects in some of the mutants indicates that non-nucleoporins are being trafficked properly when Nups are mislocalized.

  • Although the localization patterns at late time points after temperature shifting were similar, the localizations of the ER protein Kar2p and the Nups were not overlapping.


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