Example sentences for: nup

How can you use “nup” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Logarithmically growing cultures of npa1-1/sec23 and npa2-1/sec13 were shifted to the non-permissive temperature as before and then fixed and processed for IF with polyclonal antibodies against Kar2p to visualize the ER [ 45 ] . The monoclonal antibody mAb414, which recognizes a subset of Nups distinct from Nic96p and Nup170p [ 46 ] , was also used because the GFP fluorescence of the GFP-Nups was more sensitive to fixation when the GFP-Nups were mislocalized than when they remained at the nuclear rim (unpublished observation).

  • The sequence encoding GFP was fused in frame to the 3' end of the NUP170 open reading frame using the method of Baudin et al.

  • Instead, it is the sec mutants that alter ER structure that effect Nup localization.

  • In addition to the mislocalization of GFP-Nic96p and Nup170p-GFP, Nup116p, Pom152p and Nups recognized by mAb414 were also perturbed in the mutants characterized to date.

  • NIC96 is an essential nucleoporin and a member of multiple biochemically distinct NPC subcomplexes [ 29 30 ] . NUP170 , while not essential, is physically and genetically linked to a distinct subset of nucleoporins [ 31 32 33 34 ] . The rationale for using NIC96 and NUP170 are several-fold.


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