Example sentences for: nup

How can you use “nup” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • NPC clusters, NE herniations, or membrane sealing of NPCs) [ 23 31 35 ] , and in previous studies GFP-epitope tagging of Nic96p or Nup170p did not inhibit function [ [ 26 ] , our unpublished observations].

  • Cells for photography were grown in media lacking histidine, as the HIS3 linked GFP-tag of GFP-nic96 showed some instability in the nup170-GFP background.

  • Crossing a nup170-GFP:URA3 haploid isolate to SWY1695 generated doubly tagged GFP-nic96 nup170-GFP parental strains.

  • A fraction of the total cellular Sec13p is isolated in a NPC subcomplex containing Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p (a Sec13-related protein) [ 40 41 ] . Hurt and coworkers have also reported that ts sec13 mutants show enhanced growth defects when combined with a nup85Δ mutant, and result in the mislocalization of GFP-Nup49p to NE clusters and cytoplasmic foci [ 41 ] . This correlates with our observations of GFP-Nic96p and Nup170p-GFP in our sec13/npa2 mutant.

  • In the doubly tagged GFP-nic96 nup170-GFP strains, both GFP-Nic96p and Nup170p-GFP were targeted to the NE and showed punctate rim-staining characteristic of NPC localization (Figure 2).


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