Example sentences for: nucleotide-binding

How can you use “nucleotide-binding” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Recently we reported that a recombinant soluble CD39, capable of hydrolyzing both ATP and ADP, was expressed and purified from insect cells [ 25 ] . Only one nucleotide-binding site was identified on the purified soluble CD39 in the presence of Ca 2+when non-hydrolysable nucleotide analogs were used.

  • As shown in Figure 1, the degree to which the nucleotide-binding site was altered is remarkable.

  • These include the glycine-rich loop bounded by a β-strand and an a helix that is typical of Rossmann fold nucleotide-binding proteins, a conserved glutamate specific to the amino oxidase family, that interacts with ribose in FAD and other residues of the substrate and cofactor-binding site.

  • The data for both rabbit and yeast Hint enzymes indicate that the dimer's two crystallographically defined nucleotide-binding sites [ 25] bind nucleotide independently and fit well to a single submicromolar K m for each enzyme (Figure 2) [ 30].

  • Cyclic nucleotide-gated channels, for example, appear to exhibit functional dimerization of their cyclic nucleotide-binding domains [ 32 ] . The "RCK" domain of BK channels has a dimerization domain even though BK channels, too, are tetrameric at the level of the pore [ 33 ] . Finally, the GluR2 ligand-binding core has a dimerization interface in the crystal structure [ 34 ] . Evidence, including the clustering of residues involved in receptor desensitization at this interface, suggests that the dimerization is not just crystallographic but functional.


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