Words similar to nucleotide-binding
Example sentences for: nucleotide-binding
How can you use “nucleotide-binding” in a sentence? Here are some example sentences to help you improve your vocabulary:
Recently we reported that a recombinant soluble CD39, capable of hydrolyzing both ATP and ADP, was expressed and purified from insect cells [ 25 ] . Only one nucleotide-binding site was identified on the purified soluble CD39 in the presence of Ca 2+when non-hydrolysable nucleotide analogs were used.
These include the glycine-rich loop bounded by a β-strand and an a helix that is typical of Rossmann fold nucleotide-binding proteins, a conserved glutamate specific to the amino oxidase family, that interacts with ribose in FAD and other residues of the substrate and cofactor-binding site.
The data for both rabbit and yeast Hint enzymes indicate that the dimer's two crystallographically defined nucleotide-binding sites [ 25] bind nucleotide independently and fit well to a single submicromolar K m for each enzyme (Figure 2) [ 30].
Finally, catalytically inactive versions of the CYTH domain are found fused to the nucleotide kinase domain in uridine kinase homologs and may serve as an allosteric nucleotide-binding site in these enzymes (Fig.
Human Fhit protein, a member of the Fhit branch of the histidine triad superfamily of nucleotide-binding proteins, binds and hydrolyzes diadenosine polyphosphates such as ApppA and AppppA ( 1 ) into AMP plus ADP and ATP, respectively [ 8 9 10 ] . His96, which is responsible for covalent catalysis and more than 4× 10 6-fold of rate enhancement in ApppA hydrolysis [ 9 11 12 13 ] , is nonetheless dispensable for ApppA-binding and tumor suppression, suggesting that Fhit function in tumor suppression depends on formation of an E-S complex [ 2 12 ] . If Fhit-substrate complexes promote tumor suppression by stimulating a pro-apoptotic effector, then Fhit inhibitors that resemble natural substrates may promote Fhit function.
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