Example sentences for: npt

How can you use “npt” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Since the initiation codon for NPT lies upstream of the Neo 3' splice site, NTP is expressed as a fusion protein in which the first 219 amino acids of hnRNP A2/B1 are appended to codon 10 of NTP.

  • Second, since the Neo 3' splice site is downstream of the NPT initiation codon, its use may skew the targeting to favor of those genes capable of splicing upstream exons in-frame, to produce enzymatically active fusion proteins.

  • A variety of amino-terminal fusions maintain enzymatic activity (or produce enzymatically active breakdown products) including those fused to codon 12 of NPT [ 48 49 50 51 52 ] . Moreover, selection of resistant cell clones requires only minimal levels of Neo gene expression [ 53 ] . Still, genes providing the appropriate introns are expected to provide larger targets for gene trap mutagenesis than genes lacking such introns.

  • The majority of hnRNP A2/B1-Neo fusion transcripts utilised a cryptic 3' splice site within the NPT coding sequence.

  • dNTP, deoxyribonucleoside triphosphate; hnRNP heterogeneous nuclear ribonuclear protein; nt, nucleotide; LTR, long terminal repeat; MEF, mouse embryonic fibroblast; NPT, neomycin phosphotransferase; RT PCR, reverse transcriptase polymerase chain reaction.


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