Words similar to nnos

Example sentences for: nnos

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Protein 10 μg (eNOS) or 80 μg (nNOS and iNOS) were separated by SDS-PAGE under reducing conditions (1%β-mercaptoethanol) using 8-16% gradient gels (Invitrogen, Carlsbad, CA, USA).
However, iNOS protein was below the limit of detection following any level of hypoxic exposure (positive control was lipopolysaccharide-treated mouse spleen) and using several different antibodies (Affinity Bioreagents and BD Transduction Laboratories; Affinity Bioreagents blot shown), and nNOS was unchanged among the three experimental groups (positive control was mouse brain).
RNA quantity was determined using 18 S rRNA primers/probes (Applied Biosystems) and eNOS, iNOS and nNOS expression determined using the primer/probe sequences shown in Table 1. Message abundance was determined by dividing the quantity of message of interest by the quantity of 18 S ribosomal RNA.
In rat lungs, nNOS expression was increased following hypoxia [ 4], but we did not find an increase in lung nNOS in mice following hypoxia.
Sections were stained with anti-NOS antibodies (eNOS, BD Transduction Laboratories N30020, 1:400 dilution; iNOS, Zymed Laboratories 61-7000, 1:1000 dilution; nNOS, Transduction N32030, 1:500 dilution), developed with 3,3'-diaminobenzidine, and counterstained with hematoxylin.