Words similar to nnos

Example sentences for: nnos

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Protein 10 μg (eNOS) or 80 μg (nNOS and iNOS) were separated by SDS-PAGE under reducing conditions (1%β-mercaptoethanol) using 8-16% gradient gels (Invitrogen, Carlsbad, CA, USA).
eNOS = endothelial nitric oxide synthase; iNOS = inducible nitric oxide synthase; nNOS = neuronal nitric oxide synthase; NO = nitric oxide; NOS = nitric oxide synthase; PBS = phosphate-buffered saline; PCR = polymerase chain reaction; RIPA = radioimmunoprecipitation assay; RVsP = right ventricular systolic pressure.
Increased nNOS has been suggested as a compensatory vasodilator in the cerebral and coronary circulation of eNOS-deficient mice, but its role in the pulmonary circulation is unknown [ 8, 30].
Sections were stained with anti-NOS antibodies (eNOS, BD Transduction Laboratories N30020, 1:400 dilution; iNOS, Zymed Laboratories 61-7000, 1:1000 dilution; nNOS, Transduction N32030, 1:500 dilution), developed with 3,3'-diaminobenzidine, and counterstained with hematoxylin.
However, iNOS protein was below the limit of detection following any level of hypoxic exposure (positive control was lipopolysaccharide-treated mouse spleen) and using several different antibodies (Affinity Bioreagents and BD Transduction Laboratories; Affinity Bioreagents blot shown), and nNOS was unchanged among the three experimental groups (positive control was mouse brain).