Words similar to nm-myosin
Example sentences for: nm-myosin
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One new compound that will help clarify the role of nm-myosinII is blebbistatin [ 32 ] . Other small molecules wait to be found and experiments using them will continuously refine our understanding of the leading edge and the mechanism of action of these compounds.
BDM has been reported to not inhibit nm-myosinII ATPase activity [ 9 ] . We show data [Figure 1A] that supports this report.
[ 11 17 18 ] . A role for nm-myosinII may exist, given its localization and that other perturbations that affect myosin function - myosin S1 microinjection, ML-7 treatment, and expression of dominant negative nm-myosinII constructs and have similar effects to BDM treatment [ 5 7 12 ] . However, a study in chick dorsal root ganglia neurons where nm-myosinII was inactivated using chromophore assisted laser inactivation saw no effect on retrograde flow rates [ 19 ] . We know now that BDM does not in fact inhibit nm-myosinII.
Though BDM does not inhibit nm-myosinII, in permeabilized yeast ( S. cerviseae ) BDM has been shown to decrease incorporation of exogenous actin into the cytoskeleton [ 20 ] . This process of actin incorporation requires proteins involved in actin filament formation and proteins that may localize a nucleation complex [ 20 21 ] . To determine if BDM has a similar effect on actin incorporation in mammalian cells, and if so an effect on actin nucleation, spreading Swiss3T3 cells were treated briefly with a media control or 15 mM BDM and then permeabilized with the detergent saponin in the presence of fluorescently labeled actin.
BDM has recently been found not to inhibit the in vitro ATPase activity of human platelet nm-myosinII, Acanthamoeba myosinIc, human myoIe, chicken myosinV, and porcine myosinVI, though it does inhibit rabbit muscle myosinII [ 9 10 ] . Thus the literature's interpretations of BDM's effects are probably incorrect.
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