Words similar to nls
Example sentences for: nls
How can you use “nls” in a sentence? Here are some example sentences to help you improve your vocabulary:
Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).
The idea developed above that F-box protein compartmentalization contributes to substrate selection was recently confirmed directly for Cdc4p-mediated degradation of Far1p in budding yeast [ 35 ] . Both, Cdc4p and Far1p are nuclear proteins in vegetative cells, owing to the presence of nuclear localization signals (NLS).
Thus, when stable transgenic Arabidopsis plants are treated with the synthetic glucocorticoid dexamethasone (Dex), the expressed fusion proteins would be able to localize to the integrated loci by association with LacOs . This inducible expression system allows controlled expression of a small amount of GFP-LacI/NLS protein for optimal signal-to-noise and to prevent aggregation of GFP fusion proteins in the nuclei as a result of high protein concentrations.
Western blot analysis with anti-GFP polyclonal antibodies (Clonetech) was used to test inducibility of GFP-LacI/NLS in transgenic plants.
Deletion constructs, GFP-N346, GFP-N280 and GFP-N200, all contain both the NLS and the NES, but their subcellular localization patterns are strikingly different.