Words similar to nls

Example sentences for: nls

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Furthermore, we also chose to mutate a forth positively charged amino acid cluster at amino acid 358 to 360, which corresponds to the Arabidopsis COP1 NLS [ 17 ] . Since GFP-N346 exhibited clear and consistent nuclear localized pattern with speckles most resembling the full-length MmCOP1 nuclear localization pattern, it was used as the starting point for the site-directed mutagenesis studies (Fig 7A).
LacI/NLS sequence in the inserts as well as restriction sites present at variable distances in the genomic DNA neighboring the left border of the insertions.
The copy numbers of insertions in these lines were estimated with restriction digests of genomic DNA using Bgl II and Hind III in addition to Pst I, followed by Southern blot analysis using the LacI/NLS fragment as a probe (Figures 1, 2).
GFP-N280 (amino acid 71-350), which contains the RING finger and the coiled-coil domain, but not the sequence corresponding to the NLS in AtCOP1, is also solely localized in the nucleus (Fig 5Aand 5B).
However, treatment with LMB completely shifts GFP-N200 from the cytoplasm to the nucleus, implying GFP-N200 may shuttle between the nucleus and the cytoplasm, consistent with the existence of both the NES and the NLS in GFP-N200 (Fig 6and Fig 7).