Words similar to nhs-lc-biotin
Example sentences for: nhs-lc-biotin
How can you use “nhs-lc-biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and added to streptavidin-coated wells (2.
Biotinylated Ad5.CMV-LacZ, which had been treated with 15 μg/ml sulfo-NHS-LC-biotin followed by the removal of unreacted biotinylated reagent, was used to determine the immobilization efficiency of biotinylated adenoviral vectors in streptavidin-coated wells.
Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.
Sulfo-NHS-LC-biotin was used to attach biotin moieties, covalently, to the primary amino groups of poly- D-lysine on the solid surface.
The maximum infectivity was seen with Ad5.CMV-LacZ that had been treated with 15 μg/ml sulfo-NHS-LC-biotin, where the infectivity was approximately 75% of that of unmodified Ad5.CMV-LacZ used free in solution.