Example sentences for: nhs-lc-biotin

How can you use “nhs-lc-biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The maximum infectivity was seen with Ad5.CMV-LacZ that had been treated with 15 μg/ml sulfo-NHS-LC-biotin, where the infectivity was approximately 75% of that of unmodified Ad5.CMV-LacZ used free in solution.

  • After the removal of unbound Neutralite avidin, Ad5.CMV-LacZ, which had been biotinylated by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was applied to the wells and allowed to bind to unoccupied biotin-binding sites of Neutralite avidin that had been bound to biotin moieties on the solid surface.

  • Neutralite avidin (Southern Biotechnology Associates) (25 μg in 50 μl PBST per spot) was added to the spot areas within plastic rings, which had been treated with sulfo-NHS-LC-biotin, and allowed to bind to biotin moieties on the well surface.

  • First, spots of biotin moieties on the wells were created by using sulfo-NHS-LC-biotin.

  • These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.


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