Words similar to nhs-lc-biotin
Example sentences for: nhs-lc-biotin
How can you use “nhs-lc-biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Ad5.CMV-LacZ was treated at varying concentrations (0 - 100 μg/ml) of sulfo-NHS-LC-biotin on ice for 2 h in PBS (pH 7.4), followed by the removal of non-virion-associated biotinylation reagent by ultrafiltration.
Ad5.CMV-LacZ was treated with varying concentrations (0 - 30 μg/ml) of sulfo-NHS-LC-biotin, and non-virion-associated biotinylation reagent was removed by repeated ultrafiltration.
Varying numbers of biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin, were applied to streptavidin-coated wells (well diameter, 0.64 cm) for immobilization, followed by the removal of unbound viral particles.
Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.
First, spots of biotin moieties on the wells were created by using sulfo-NHS-LC-biotin.
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