Words similar to nhs-lc-biotin
Example sentences for: nhs-lc-biotin
How can you use “nhs-lc-biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Sulfo-NHS-LC-biotin was used to attach biotin moieties, covalently, to the primary amino groups of poly- D-lysine on the solid surface.
Biotinylation of Ad5.CMV-LacZ was performed by using sulfo-NHS-LC-biotin (Pierce Chemical, Rockford, IL), a biotin derivative containing an N -hydroxysuccinimidyl ester (NHS) that reacts with primary amino groups.
In contrast, substantial numbers of D-17 cells were transduced when Ad5.CMV-LacZ was treated with 3 - 30 μg/ml sulfo-NHS-LC-biotin.
These spot areas within plastic rings were treated with 1 mg/ml sulfo-NHS-LC-biotin in PBS (pH 7.4) (50 μl per spot) for 2 h at 4°C and then washed with PBST to remove unreacted biotinylation reagent.
Varying numbers of biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin, were applied to streptavidin-coated wells (well diameter, 0.64 cm) for immobilization, followed by the removal of unbound viral particles.
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