Words similar to nhs-lc-biotin

Example sentences for: nhs-lc-biotin

How can you use “nhs-lc-biotin” in a sentence? Here are some example sentences to help you improve your vocabulary:

Sulfo-NHS-LC-biotin was used to attach biotin moieties, covalently, to the primary amino groups of poly- D-lysine on the solid surface.
Biotinylated Ad5.CMV-LacZ (1 × 10 7viral particles per spot), prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, were incubated in the spot areas within plastic rings at 25°C for 2 h, followed by washing the wells with PBST to remove unbound viral particles.
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
In contrast, substantial numbers of D-17 cells were transduced when Ad5.CMV-LacZ was treated with 3 - 30 μg/ml sulfo-NHS-LC-biotin.
When Ad5.CMV-LacZ was treated with sulfo-NHS-LC-biotin at a concentration of 0.6 μg/ml or lower, almost no infection of D-17 cells was seen.