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Example sentences for: nhbes

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3Ashows the results of a western blot of whole-cell extracts from NHBEs exposed to increasing concentrations of CSC and probed for phosphorylated ERK1/2, the activated form of ERK1/2.
To determine the effects of CSC on cell viability, A549 cells and NHBEs were exposed to increasing concentrations of CSC for 30 min then examined using the trypan blue dye-exclusion test (Fig.
One of the important observations of this study is the effect of CSC on NHBEs.
A549 cells or NHBEs were grown to approximately 75% confluence in 12-well plates, treated with various concentrations of CSC in 0.5% DMSO, and total RNA was isolated using RNeasy method (Qiagen, Valencia, CA, USA).
To confirm the results of immunoblot detection of phosphorylated ERK1/2, NHBEs were exposed to 0.4 μg/ml CSC for 30 min, then fixed and stained for phosphorylated ERK1/2.