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Example sentences for: nhbes

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In essence, these results demonstrate that NHBEs respond to moderate doses of CSC by activating both the ERK1/2 and the NFκB pathways, and by upregulating the expression of several genes encoding proinflammatory molecules.
One of the important observations of this study is the effect of CSC on NHBEs.
IL-1β, which was significantly upregulated in NHBEs following CSC exposure, has been shown to be important for activation of IL-8 [ 6 ] and ICAM-1 [ 4 ] . This is also consistent with the report that epithelial cells regulate inflammatory events by secreting cytokines and chemokines such as IL-1β, IL-8, GM-CSF, TNF-α, and sICAM-1 [ 2 ] . These paracrine effectors can then produce further primary local inflammation or amplify the effects of previously activated macrophages, eosinophils, mast cells, or lymphocytes.
A549 cells or NHBEs were grown to approximately 75% confluence in 12-well plates, treated with various concentrations of CSC in 0.5% DMSO, and total RNA was isolated using RNeasy method (Qiagen, Valencia, CA, USA).
A significant finding of this study is that MAPKs such as ERK1/2 (p44/p42) are consistently activated in NHBEs.