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Example sentences for: nhbes

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IL-1β, which was significantly upregulated in NHBEs following CSC exposure, has been shown to be important for activation of IL-8 [ 6 ] and ICAM-1 [ 4 ] . This is also consistent with the report that epithelial cells regulate inflammatory events by secreting cytokines and chemokines such as IL-1β, IL-8, GM-CSF, TNF-α, and sICAM-1 [ 2 ] . These paracrine effectors can then produce further primary local inflammation or amplify the effects of previously activated macrophages, eosinophils, mast cells, or lymphocytes.
Primary cultures of NHBEs (Clonetics, San Diego, CA, USA) were obtained at second passage and cultured according to the manufacturer's instructions using the media and growth factors supplied with the cells.
Cultures of A549 cells or NHBEs were grown on 8-well slides and treated with CSC as described above, fixed with 4% paraformaldehyde for 15 min at room temperature, then examined by fluorescent microscopy.
One of the important observations of this study is the effect of CSC on NHBEs.
Total RNA isolated from NHBEs following exposure to CSC was subjected to RT-PCR, and the results are shown in Fig.