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Example sentences for: nhbes

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IL-1β, which was significantly upregulated in NHBEs following CSC exposure, has been shown to be important for activation of IL-8 [ 6 ] and ICAM-1 [ 4 ] . This is also consistent with the report that epithelial cells regulate inflammatory events by secreting cytokines and chemokines such as IL-1β, IL-8, GM-CSF, TNF-α, and sICAM-1 [ 2 ] . These paracrine effectors can then produce further primary local inflammation or amplify the effects of previously activated macrophages, eosinophils, mast cells, or lymphocytes.
For immunocytochemistry, NHBEs grown on 8-well chamber slides were exposed to CSC at a concentration of 0.4 μg/ml for 30 min.
However, with increasing concentration of CSC, NHBEs showed as high as 20% apoptosis, while A549 cells remained unchanged.
To examine if CSC-upregulation of cytokine production involves activation of MAPKs we examined CSC-treated NHBEs for specific phosphorylated species.
To confirm the results of immunoblot detection of phosphorylated ERK1/2, NHBEs were exposed to 0.4 μg/ml CSC for 30 min, then fixed and stained for phosphorylated ERK1/2.