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Example sentences for: nh
How can you use “nh” in a sentence? Here are some example sentences to help you improve your vocabulary:
At the end the unbound radiolabelled iron was removed by chromatography using sephadex G-10 as described by Rosenberg [ 44 ] . The uptake of radiolabelled iron siderophore was studied by the method described by Rosenberg [ 44 ] . Briefly A. baumannii was grown in CDM-Fe medium also in CDM+Fe medium to a mid exponential phase and was washed twice with uptake medium (30 mM Na 2 HPO 4 , 10 mM K 2 HPO 4 , 10 mM (NH 4 ) 2 SO 4 ,1 mM MgCl 2 and 40 μM CaCl 2 pH 6.9) at 4°C and re-suspended in the same medium at a density of A 470 = 0.4.
The MAP kinase pathway consists of 3 major parallel pathways designated as the ERK (p42/44, Extracellular signal-Regulated Kinase), SAPK/JNK (p46, Stress-Activated Protein Kinase/ c-Jun NH2-terminal Kinase) and p38 pathways [ 12 13 14 ] . Activation of each pathway above involves phosphorylation of a number of upstream and downstream family members of the pathway.
Following isolation of pure plaques, the insert DNAs were prepared by PCR methods with the following conditions: 100 μl reactions containing 1X Pfu buffer (20 mM Tris-Cl, pH 8.75, 10 mM KCl, 2 mM MgSO 4 , 10 mM (NH 4 ) 2 SO 4 , 0.1% v/v Triton X-100, 100 μg/ml bovine serum albumin), 1 μM λgt11 forward and reverse primers (Promega, Madison, WI), 0.2 mM deoxyribonucleotides, and 5 Units of recombinant Pfu DNA Polymerase (Stratagene, La Jolla, CA).
Cellular response to chronic TLK286 exposure included a two-fold decrease in GSTP1-1 protein levels, confirming a GSTP1-1 mediated mechanism of activation [ 7 ] . Mouse embryo fibroblast cells from GSTP1-1 deficient animals elevated the expression of signal-regulated kinases ERK1/ERK2 and reduced the doubling time of cell proliferation [ 8 ] . That paper also found that a clone of HL60 cells resistant to TLK199, an inhibitor of GSTP1-1, has been shown to have elevated activities of c-Jun NH 2 -terminal kinase (JNK) and ERK1/ERK2.
Following incubation with secondary antibodies, sections were washed three times for ten minutes in PBS and mounted on RITE-ON micro slides (Becton, Dickinson and Company, Portsmouth, NH) using Flouromount-G (Southern Biotechnology Associates, Inc., Birmingham, AL, cat# 0100-01) and coverslipped (VWR Scientific, Media, PA).
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