Example sentences for: nestin

How can you use “nestin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Polyclonal antibodies used were MyoD (Santa Cruz Biotechnology, Santa Cruz, California, United States) and nestin (gift from R. McKay); monoclonal antibodies were vimentin, alpha smooth muscle actin, fast-switch myosin, pan-cytokeratin (all from Sigma), and human nuclear antigen (Chemicon, Temecula, California, United States).

  • After 2 wk at stage 2, NS-derived cells remained in clusters (see Figure 1A), but Nestin and Ki67 were expressed in fewer than 35% of cells (see Figure 2), indicating possible differentiation of cells toward lineage-restricted fates.

  • Chenn and Walsh recently created transgenic mice in which β-catenin stabilized by an amino-terminal truncation was expressed in subventricular zone precursor cells in utero under control of the nestin promoter [ 22 ] . Brains from these transgenic embryos contained increased numbers of periventricular neuroepithelial cells because a greater number of the precursors re-entered the cell cycle after mitosis.

  • In stage 3 cultures, Nestin and Ki67 expression was nearly extinguished (see Figure 2), while expression of En1, ngn3, and Hb9 increased (see Figure 1B and 1C).

  • Second, our immunohistochemical analysis shows that stage 4 insulin-expressing cells do not express markers like Nestin, β-tubulin III, MAP2, or Olig2, suggesting that these insulin + cells are non-neuronal, but we cannot formally rule out the alternate possibility that our methods produced neural cells capable of secreting insulin.


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