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Example sentences for: nestin

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For quantification of cells expressing Nestin, Ki67, β-tubulin III, activated caspase-3, insulin, or C-peptide, NS-derived cell clusters were fixed, and sectioned to generate 7-μm-thick tissue sections.
Polyclonal antibodies used were MyoD (Santa Cruz Biotechnology, Santa Cruz, California, United States) and nestin (gift from R. McKay); monoclonal antibodies were vimentin, alpha smooth muscle actin, fast-switch myosin, pan-cytokeratin (all from Sigma), and human nuclear antigen (Chemicon, Temecula, California, United States).
In stage 3 cultures, Nestin and Ki67 expression was nearly extinguished (see Figure 2), while expression of En1, ngn3, and Hb9 increased (see Figure 1B and 1C).
Chenn and Walsh recently created transgenic mice in which β-catenin stabilized by an amino-terminal truncation was expressed in subventricular zone precursor cells in utero under control of the nestin promoter [ 22 ] . Brains from these transgenic embryos contained increased numbers of periventricular neuroepithelial cells because a greater number of the precursors re-entered the cell cycle after mitosis.
Isolated human neural progenitors spontaneously aggregate in these conditions to produce a cell cluster called a NS, and 85%–90% of NS cells produce the intermediary filament Nestin (Figures 1 and 2), a putative marker of neural stem and progenitor cells [19].