Words similar to nestin
Example sentences for: nestin
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For quantification of cells expressing Nestin, Ki67, β-tubulin III, activated caspase-3, insulin, or C-peptide, NS-derived cell clusters were fixed, and sectioned to generate 7-μm-thick tissue sections.
Second, our immunohistochemical analysis shows that stage 4 insulin-expressing cells do not express markers like Nestin, β-tubulin III, MAP2, or Olig2, suggesting that these insulin + cells are non-neuronal, but we cannot formally rule out the alternate possibility that our methods produced neural cells capable of secreting insulin.
Insulin + cells showed little or no co-expression of neural stem cell markers like Nestin (see Figure 1), or other markers of neural lineages known to develop from human neural progenitors, like MAP2 (differentiated neurons; Figure 5D–5F), Olig2 (multipotent precursors, bipotent glial precursors, and oligodendrocytes; Figure 5J–5L; see [38]), or myelin basic protein (data not shown).
After 2 wk at stage 2, NS-derived cells remained in clusters (see Figure 1A), but Nestin and Ki67 were expressed in fewer than 35% of cells (see Figure 2), indicating possible differentiation of cells toward lineage-restricted fates.
In stage 3 cultures, Nestin and Ki67 expression was nearly extinguished (see Figure 2), while expression of En1, ngn3, and Hb9 increased (see Figure 1B and 1C).