Example sentences for: nested

How can you use “nested” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Amplified products were diluted 10-fold in sterile water and 1 μl of diluted primary PCR products were added to 24 μl of secondary PCR master mix containing nested primers, 1 and 2R, to ensure specific amplification of double-stranded templates containing both adaptors.

  • Five identified single nucleotide polymorphisms, c. 901C>G (exon 6), c. 1197A>G (exon 9), c. 1737A>G (exon 12), c. 2286A>G (exon 16), and c. 5002A>G (exon 34), were also analyzed following 2 rounds of nested PCR; the exon specific forward and reverse primers have been previously described [ 32 33 ] . The exons containing these polymorphisms were PCR amplified from genomic DNA using PCR conditions identical to those described above.

  • "Nested" units in a large or complex case study (note that the instance or unit must be specified and data appropriate to it collected).

  • In addition, a nested ANOVA using fitness in LB revealed highly significant variation among populations but not within populations (Table 4).

  • Polymerase chain reaction (PCR) products (2 μ l) generated from either genomic DNA or cDNA were amplified using 5 pmol of nested sense V H family-specific FR3 primer and radiolabeled antisense nested J H consensus primer that had been end-labeled with γ 32-P (New England Nuclear, Beverly, MA, USA) using T4 polynucleotide kinase (Promega, Madison, WI, USA).


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