Words similar to negative-strand
Example sentences for: negative-strand
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The minimal constituents of a nonsegmented negative-strand RNA viral RdRP are the viral large protein L and the phosphoprotein P [ 23 ] . While the L protein is believed to possess the basic RNA polymerase function, the P protein serves as its transcription factor.
Viral movement proteins (MPs) are encoded by diverse, unrelated families of plant viruses, such as positive-strand RNA, negative-strand RNA, single-stranded DNA and double-stranded DNA viruses, and are essential for cell-to-cell movement of all these viruses [ 31, 32].
RSV negative-strand genomic RNA and N and F mRNA from infected cells were quantified by real-time RT-PCR using specific primers described previously [ 25 ] and a Bio-Rad iCycler iQ Real-time PCR Detection System (Bio-Rad Laboratories, Hercules, CA).
The RNA genome of RSV is about 15 kb long and contains 11 documented protein-coding genes [ 13 ] . Three viral proteins are minimally required to reconstitute the functional transcription complex of NNR viruses [ 3 ] : the nucleocapsid protein (N) that wraps the negative-strand genome RNA and its full-length complement, the positive-strand antigenome RNA, thus converting them into highly nuclease-resistant, chromatin-like templates; the large protein (L), which is the major subunit of the RdRP; and the phosphoprotein (P), which is the smaller subunit of RdRP and an essential transcription factor of L [ 19 20 21 22 ] . In RSV, optimal transcription, although not replication, additionally requires the transcription antitermination protein M2-1 [ 13 ] . In addition, cellular actin, and to a lesser extent, profilin, are also required for viral transcription [ 17 18 ] .
To answer these questions, we have used a nonsegmented negative-strand RNA (NNR) virus as a test target for RNA-mediated inhibition.
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