Words similar to ndei

Example sentences for: ndei

How can you use “ndei” in a sentence? Here are some example sentences to help you improve your vocabulary:

pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).
3. This model was subsequently confirmed in two ways: an NdeI restriction site predicted from Southern blots was confirmed in the flanking sequence [ 27 ] ; and PCR amplification with specific primers across the flanks of the transgene and across the internal LB-RB junction resulted in the expected product lengths (Fig.
We used genomic DNA for a template, and for each mutant transcribed the complete ftsZ sequence (adding NdeI and BamHI cloning sites at the ends) using error-correcting Pfu polymerase [ 37] and outward-facing primers containing the desired mutations.
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.