Words similar to ndei
Example sentences for: ndei
How can you use “ndei” in a sentence? Here are some example sentences to help you improve your vocabulary:
3. This model was subsequently confirmed in two ways: an NdeI restriction site predicted from Southern blots was confirmed in the flanking sequence [ 27 ] ; and PCR amplification with specific primers across the flanks of the transgene and across the internal LB-RB junction resulted in the expected product lengths (Fig.
The PCR product was digested with Xba I and Mro I and ligated into pBS-KS +-NFATp that had been digested with Xba I and Mro I. pVL1392-HAX-NFATp(1-722) was made by subcloning a Nde I - Msc I fragment from pBS-KS +-NFATp(NdeI) into the Nde I and Sma I sites of pVL1392-HAX.
We used genomic DNA for a template, and for each mutant transcribed the complete ftsZ sequence (adding NdeI and BamHI cloning sites at the ends) using error-correcting Pfu polymerase [ 37] and outward-facing primers containing the desired mutations.
pEGFP-N346 was generated by inserting a HindIII-NdeI fragment (containing amino acid 70-416 of MmCOP1; NdeI site was filled in by Klenow DNA polymerase) into pEGFP vector digested by HindIII and SmaI.
pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).