Words similar to nde

Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

An Nde I- Bam HI fragment of a cDNA clone with wild-type rec12 +sequence (see above) was sub-cloned into the pREP42 vector to generate pREP42- rec12 + cDNA . The integrity of all clones was determined by a combination of restriction mapping, PCR, and DNA sequencing.
The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.
The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.
The forward primer (5'-GTTATT CATATG ATTCGTCATATCGCCATT-3') was designed to introduce an Nde I restriction site ( CATATG ) overlapping the start codon.
The PCR products were gel-purified using low melting temperature agarose and subjected to restriction enzyme digestion with Nde I and Xho I. The same two restriction enzymes were used to digest plasmid pET-24b+ DNA.