Words similar to nde

Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.
After confirmation of the sequence, the mutated pL1PUC19 plasmid was digested with Nde I and Hind III, and the 900 bp, mutated L1 gene was gel purified and ligated into pET26b to create the mutant overexpression plasmids.
“Our experiments indicate that the loss of Nde1 causes neurons to mature prematurely.
pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).
A dsDNA oligonucleotide linker, containing cohesive Nde I and Eco RI ends, was constructed (5'-TATGAGCCATCATCATCATCATCATAGCATG-3' and 5'-AATTCATGCTATGATGATGATGATGATGGCTAC-3').