Words similar to nde

Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

The PCR product was then cloned into the pET15b vector which carries an N-terminal Histag sequence followed by BamH1 and Nde 1 cloning sites (Novagen Inc., Madison, WI).
After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.
The PCR products were gel-purified using low melting temperature agarose and subjected to restriction enzyme digestion with Nde I and Xho I. The same two restriction enzymes were used to digest plasmid pET-24b+ DNA.
A dsDNA oligonucleotide linker, containing cohesive Nde I and Eco RI ends, was constructed (5'-TATGAGCCATCATCATCATCATCATAGCATG-3' and 5'-AATTCATGCTATGATGATGATGATGATGGCTAC-3').
The pce1::kanMX cassette was excised from PCE1Δ by digestion with Aat II and Nde I. The cassette fragments were gel-purified and then used to transform diploid S. pombe.