Words similar to nde

Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

To prepare hexa-histidine amino terminally-tagged RGS3T, we modified RGS3T cDNA [ 24 ] using PCR with primers containing restriction sites (the 5' primer incorporated a Bam HI site, whereas the 3' primer bore an Nde 1 site).
A dsDNA oligonucleotide linker, containing cohesive Nde I and Eco RI ends, was constructed (5'-TATGAGCCATCATCATCATCATCATAGCATG-3' and 5'-AATTCATGCTATGATGATGATGATGATGGCTAC-3').
The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
“Our experiments indicate that the loss of Nde1 causes neurons to mature prematurely.