Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The 5' and 3' primers contained Nde I and Bam HI restriction sites, respectively, and were designed to amplify the rec12 +cDNA from the first ATG in exon 1 to a position +142 bp downstream of the stop codon in exon 5. RT-PCR products were cloned by blunt-end ligation into pCR-Blunt (Invitrogen Corp.) and both strands were subject to DNA sequencing (GenBank accession no.

  • In the second step, the Nde I EcoR I fragment from pBS-NFATp(391-583) was ligated into the Nde I and EcoR I sites of pVL1392-GST.

  • The forward primer (5'-GTTATT CATATG ATTCGTCATATCGCCATT-3') was designed to introduce an Nde I restriction site ( CATATG ) overlapping the start codon.

  • “Our experiments indicate that the loss of Nde1 causes neurons to mature prematurely.

  • A region of NFATp spanning the translational start site ATG and an internal Mro I restriction site was amplified by PCR, creating a product with an Nde I site at the translational start site ATG as well as an upstream Xba I site.


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