Words similar to nde

Example sentences for: nde

How can you use “nde” in a sentence? Here are some example sentences to help you improve your vocabulary:

The PCR product was then cloned into the pET15b vector which carries an N-terminal Histag sequence followed by BamH1 and Nde 1 cloning sites (Novagen Inc., Madison, WI).
A region of NFATp spanning the translational start site ATG and an internal Mro I restriction site was amplified by PCR, creating a product with an Nde I site at the translational start site ATG as well as an upstream Xba I site.
pVL-GST-NFATp was created by subcloning a Nde I - EcoR I fragment containing the NFATp cDNA from pBS-KS +-NFATp(NdeI) into the Nde I and EcoR I sites of pVL1392-GST (gift of S. Ruppert and R. Tjian).
After digestion with Nde I and BamH I the coding sequence was subcloned to the vector pET-11a (Stratagene) for gene expression experiments.
After confirmation of the sequence, the mutated pL1PUC19 plasmid was digested with Nde I and Hind III, and the 900 bp, mutated L1 gene was gel purified and ligated into pET26b to create the mutant overexpression plasmids.