Example sentences for: nbt
How can you use “nbt” in a sentence? Here are some example sentences to help you improve your vocabulary:
Nitro blue tetrazolium (NBT), 5-bromo-4-chloroindol-3-yl phosphate (BCIP) and poly(vinylidene difluoride) (PVDF) were from Bio-Rad.
The immunolabeled bands were visualized with alkaline-phosphatase conjugated secondary antibody using NBT/BCIP enzyme substrate (Chemicon).
Sequential immunoaffinity columns were used to purify β-tubulin classes II and III with their associated α-tubulins as described previously [ 12 ] . Known amounts of PC-tubulin, β-tubulin classes II and III, and pig brain samples ( n = 12) from the cerebellum and right cerebrum were examined by SDS-polyacrylamide-gel electrophoresis and transferred to PVDF filters [ 43 ] . Alkaline phosphatase colorimetric reactions (NBT and BCIP) were used to identify tubulin isotypes.
Cells were pipetted onto a Bright Line counting chamber and scored as positive for differentiation by the presence of purple NBT precipitate using bright field microscopy.
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.