Words similar to nbt

Example sentences for: nbt

How can you use “nbt” in a sentence? Here are some example sentences to help you improve your vocabulary:

BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Following continuous exposure to 1 μM retinoic acid for 96 hours, almost 40% of cells have differentiated to granulocytes, as seen by the positive NBT staining.
Slides were then washed twice for 15 min in TBS, and equilibrated for 5 min in TBS pH 9.5 (100 mM Tris, 100 mM NaCl, 50 mM MgCl 2 ). They were then stained in NBT/BCIP solution (Pierce, Cat No.
Cells were pipetted onto a Bright Line counting chamber and scored as positive for differentiation by the presence of purple NBT precipitate using bright field microscopy.
Following nine 10-min washes in 0.1% Tween 20 in PBS and two rinses in AP buffer (50 mM MgCl 2 , 100 mM NaCI, 100 mM Tris pH 9.5, 0.01% Tween 20) the NBT/BCIP color substrates were used to detect the hybridized probes.