Words similar to narl-phosphate

Example sentences for: narl-phosphate

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Therefore, a simple model that accounts for the footprint data is the assembly of multiple NarL-phosphate molecules onto one face of the DNA that somehow protect the minor groove from hydroxyl radical attack [ 28 29 30 ] . In an alternative model, NarL binds only at the three proposed NarL heptamer consensus sites spaced at 20 bp intervals within the dmsA regulatory region (Figure 4).
The size of the protected region did not change further when up to five-fold higher amounts of NarL-phosphate were used (10 μM, data not shown).
When examined by DNase I footprinting analysis, the altered dmsA Fragment B (Figure 5, lanes 6 to 9) revealed a 38 bp NarL-phosphate protected region extending from position -51 to -13.
When NarL-phosphate was incubated with the coding strand of DNA, a 76 bp region was protected that extends from position -48 to +28 relative to the start of dmsA transcription (Figure 2, open rectangle).
For the non-coding DNA strand, the size of the DNase I footprint pattern appears to increase when higher levels of NarL-phosphate are present.