Words similar to narl-phosphate
Example sentences for: narl-phosphate
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This signifies that NarL-phosphate binds to only one side of the DNA molecule dmsA promoter region [ 27 28 29 30 ] . The hydroxyl radical data are also consistent with the binding of multiple molecules of NarL-phosphate to the dmsA promoter region as suggested by the DNase I data.
By either of the above models, the location of the NarL-phosphate protected regions suggests that NarL may compete with Fnr and/or RNA polymerase for occupancy on the DNA but only when the bacteria are grown anaerobically in the presence of nitrate, conditions where NarL is in the activated form.
Furthermore, non-phosphorylated NarL gave no DNase I protections in the dmsA promoter region under the same conditions for either strand (up to 10 μM), suggesting that NarL-phosphate, but not NarL, can act as a transcriptional repressor for dmsA expression.
The size of the protected region did not change further when up to five-fold higher amounts of NarL-phosphate were used (10 μM, data not shown).
Finally, a similar hydroxyl radical footprint pattern of 8-9 protected regions of 3-4 bp spaced 10 nucleotides apart was also observed for NarL-phosphate at the promoter region of the
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