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Example sentences for: narl-phosphate

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Finally, a similar hydroxyl radical footprint pattern of 8-9 protected regions of 3-4 bp spaced 10 nucleotides apart was also observed for NarL-phosphate at the promoter region of the
Therefore, a simple model that accounts for the footprint data is the assembly of multiple NarL-phosphate molecules onto one face of the DNA that somehow protect the minor groove from hydroxyl radical attack [ 28 29 30 ] . In an alternative model, NarL binds only at the three proposed NarL heptamer consensus sites spaced at 20 bp intervals within the dmsA regulatory region (Figure 4).
The protections are consistent with the proposal that NarL-phosphate recognizes and binds at multiple heptamer recognition sites within the dmsA P1 promoter region.
When examined by DNase I footprinting analysis, the altered dmsA Fragment B (Figure 5, lanes 6 to 9) revealed a 38 bp NarL-phosphate protected region extending from position -51 to -13.
This signifies that NarL-phosphate binds to only one side of the DNA molecule dmsA promoter region [ 27 28 29 30 ] . The hydroxyl radical data are also consistent with the binding of multiple molecules of NarL-phosphate to the dmsA promoter region as suggested by the DNase I data.