Words similar to narl-phosphate

Example sentences for: narl-phosphate

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The protections are consistent with the proposal that NarL-phosphate recognizes and binds at multiple heptamer recognition sites within the dmsA P1 promoter region.
To establish if the entire NarL protected region is required for NarL-phosphate to bind DNA, a DNA fragment (designated Fragment B, Figure 5) containing a truncated region of the dmsA regulatory sequence was constructed.
When NarL-phosphate was incubated with the coding strand of DNA, a 76 bp region was protected that extends from position -48 to +28 relative to the start of dmsA transcription (Figure 2, open rectangle).
The NarL interactions with the dmsA promoter region occurred at ten bp intervals and were offset by 3 bp in the 3' direction, suggesting the assembly of multiple NarL-phosphate molecules onto one face of the DNA that protect the minor groove.
The size of the protected region did not change further when up to five-fold higher amounts of NarL-phosphate were used (10 μM, data not shown).