Words similar to narl-phosphate

Example sentences for: narl-phosphate

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When examined by DNase I footprinting analysis, the altered dmsA Fragment B (Figure 5, lanes 6 to 9) revealed a 38 bp NarL-phosphate protected region extending from position -51 to -13.
Additional NarL-phosphate monomers then assemble on the DNA to form a stable DNA protein complex.
The protections are consistent with the proposal that NarL-phosphate recognizes and binds at multiple heptamer recognition sites within the dmsA P1 promoter region.
Furthermore, non-phosphorylated NarL gave no DNase I protections in the dmsA promoter region under the same conditions for either strand (up to 10 μM), suggesting that NarL-phosphate, but not NarL, can act as a transcriptional repressor for dmsA expression.
However, a somewhat weaker binding of NarL-phosphate to the DNA fragment containing the truncated dmsA region relative to the full-length region may suggest that NarL binds at the promoter in a weakly cooperative fashion.