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Example sentences for: narl

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To establish if the entire NarL protected region is required for NarL-phosphate to bind DNA, a DNA fragment (designated Fragment B, Figure 5) containing a truncated region of the dmsA regulatory sequence was constructed.
Hydroxyl radical footprinting of the NarL interactions with dmsA promoter DNA was also performed for both strands of DNA (Figure 2and 3).
Hydroxyl radical footprinting at the dmsA promoter was performed as described [ 27 ] . NarL binding to the indicated DNA fragment was performed as described above, omitting glycerol from the binding buffer.
As the three consensus NarL boxes flank the dmsA promoter and are spaced 20 bp apart (Figure 4), the spacing and orientation of the NarL protected regions make it tempting to speculate that NarL-phosphate binds at each site.
The region of dmsA promoter DNA protected by the NarL regulatory protein was identified by DNase I and hydroxyl radical footprinting experiments.