Words similar to myoglobin
Example sentences for: myoglobin
How can you use “myoglobin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Quantification of the relative amount of rhod-2 in the heart using absorbance measurements, was done by taking the ratio of absorbance at 524 nm (rhod-2 sensitive) to 589 nm (rhod-2 insensitive) which eliminated the effect of motion as both wavelengths would be equally affected by motion, though only 524 reflected the concentration of rhod-2 [ 7 ] . These wavelengths (524, 589 nm) were chosen as these were isosbestic points not effected by changes in absorbance of myoglobin induced by oxygen desaturation [ 7 ] . In solution maximal rhod-2 absorbance is at 554 nm.
These early observations formed the basis of the classical terminology of red and white muscle fibers, which was subsequently found to be related to myoglobin (an iron-containing oxygen-transport protein in the red cells of the blood) content (Needham 1926).
The mass calibration was performed using heart myoglobin (Sigma)
A max is not determined when heart has tetanized because of the marked influence of the shape change and desaturation of myoglobin on the reflectance spectrum.
where K d is the dissociation constant for rhod-2 and calcium (determined by in-vitro calibration with rhod-2 and myoglobin by del Nido et al, ref [ 20 ] , and confirmed by in-vivo manganese quenching, ref [ 29 ] ) and is 710 nM, F t = fluorescence at time t, F max =maximal fluorescence from tetanized heart, and the fluorescence from the heart assuming rhod-2 had no calcium bound is given by F 0 = F b + a (F max - F b ), where F b is the background counts from the heart prior to dye loading, and a = rhod-2 fluorescence in the absence of calcium / rhod-2 fluorescence in the presence of saturating calcium.
Loading...