Words similar to myoc

Example sentences for: myoc

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Cloning of rabbit MYOC
Primers derived from conserved regions of human and mouse MYOC (Table 1, primers Exon1F/Exon1R) were used to amplify a 250-bp section of rabbit MYOC exon 1 (Fig.
Disease-causing myocilin mutants are less soluble in Triton [ 16 ] and are retained by the cell [ 12 ] . Other studies have shown that a truncated form of myocilin does not get secreted [ 20 21 ] and that mouse MYOC knockouts are without discernable phenotype [ 42 ] . Furthermore, a human hemizygous individual containing an interstitial deletion of chromosome 1, which encodes MYOC , was without evidence of glaucoma [ 43 ] and another patient homozygous for a severly truncated form of myocilin had no evidence of glaucoma [ 44 ] or other discernable phenotypes.
Rabbit MYOC encodes a protein highly similar to human MYOC . Rabbit myocilin is secreted and is detected in rabbit aqueous humor.
PCR products were restriction digested with BamH1 (exon 1) or Not1 (exon 2), purified from an agarose gel by Qiaquick extraction (Qiagen), and simultaneously subcloned into a BamH1/Not1 digested pcDNA3 (Invitrogen) vector to create a directional clone of rabbit MYOC exons 1 and 2 spliced together (pcDNA3.RbMYOCex1-2).