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Example sentences for: mrc-

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MRC-5 fibroblast infection of supernatants from CMV superinfections
The strain is a derivative of VL6-48 with the ura3-52 gene replaced by the KanMX cassette [ 15 ] . Spheroplasts were generated as described previously [ 29 ] . Agarose plugs (60 ul) containing approximately 2 ug of high molecular weight DNA were prepared from normal human fibroblasts MRC-5 (ATCC) or from liver cells of the Tg.AC mouse [ 11 ] . Linearized TAR cloning vector (1 ug) was added to the plugs, treated with agarase, and mixed with yeast spheroplasts.
with minor modifications [ 13 ] as reported previously [ 17 ] . Briefly, six serial 10-fold dilutions of virus were inoculated onto MRC-5 cells in the absence of antiviral drug or in the presence of either ACV or PCV at a concentration of antiviral 10 times higher than the average IC 50 for wild type parental HSV-1 and HSV-2, respectively.
CMV infected and uninfected MRC-5 fibroblasts were used as positive controls for viable CMV and antibody staining.
PRAs were performed in MRC-5 cells as described previously [ 17 ] . The plating efficiency of the virus preparations generated in vivo was determined according to the method developed by Hall et al.