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Example sentences for: mrc-

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CMV infected and uninfected MRC-5 fibroblasts were used as positive controls for viable CMV and antibody staining.
CMV was grown and harvested from MRC-5 fibroblast cultures (Biowhittaker, Walkersville, MD) and concentrated by ultra-centrifugation (28000 g for 60 minutes at 4°C).
Notably, whereas the four HSV-2 parental viruses had spontaneous mutation frequencies significantly higher than HSV-1 isolates (Table 1), the frequency of errors from pol -recombinant viruses expressing an HSV-2 Pol in an HSV-1 background were indistinguishable from HSV-1 SC16 when tested in MRC-5 cells.
Vero (ATCC, passage 15-20), MRC-5 (ATCC, passage 12-15) and PolB3 (kind gift of D. Coen, Harvard University, Boston MA) cell lines were grown in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal calf serum (FCS) and incubated at 37°C, 5%CO 2 . PolB3 represents a Vero cell line constitutively expressing the HSV-1 Pol [ 17 ] . PCV was synthesized at SmithKline Beecham Pharmaceuticals.
The strain is a derivative of VL6-48 with the ura3-52 gene replaced by the KanMX cassette [ 15 ] . Spheroplasts were generated as described previously [ 29 ] . Agarose plugs (60 ul) containing approximately 2 ug of high molecular weight DNA were prepared from normal human fibroblasts MRC-5 (ATCC) or from liver cells of the Tg.AC mouse [ 11 ] . Linearized TAR cloning vector (1 ug) was added to the plugs, treated with agarase, and mixed with yeast spheroplasts.