Words similar to monocyte-derived
Example sentences for: monocyte-derived
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To enrich for clones with increased survival in human macrophages, the library was passed through THP-1 macrophages as shown schematically in Figure 1. THP-1 is a human monocyte-derived macrophage cell line [ 21 ] and wild-type M. smegmatis bacteria are rapidly killed by THP-1 macrophages [ 22 ] . After three rounds of enrichment, the resulting clones were analyzed individually by Southern blot to evaluate the number of different clones present as previously described [ 20 ] . Out of the 3000 colonies recovered, genomic DNAs from the bacteria of 90 randomly chosen colonies were digested with Pst I and hybridized with the pYUB178 vector.
In the studies reported here, we used a similar enrichment scheme to identify two additional recombinant M. smegmatis clones, sur2 and sur3, that demonstrated enhanced survival during infections of THP-1 human monocyte-derived macrophages.
Interestingly, although lamina propria of ectocervix showed the presence of primitive (CD14) moderately (HLA-DR) and well differentiated MDC (CD68), the monocyte-derived cells entering epithelium appear to initiate a new differentiation associated with CD1a expression and HLA-DR and CD68 secretion.
Details of the experimental, RNA preparation, and GeneChip hybridization procedures, along with details of the chip-to-chip normalizations and statistical analysis of differential gene expression are provided in Cicala et al . [ 10 ] . Briefly, primary human PBMCs and monocyte-derived macrophages were incubated for 16 hours with HIV-1 envelope protein (gp120).
Genes identified using enrichment or screening protocols include Rv2962c and Rv2958c (probable glucuronsyl transferases), Rv2220 (glutamine synthetase A1), Rv3913-Rv3914 (thioredoxin, thioredoxin reductase) and Rv2416c ( eis, unknown function) [ 14 15 22 29 ] . By screening insertional mutants of M. smegmatis, Lagier et al [ 30 ] isolated 8 mutants with impaired ability to survive in human peripheral blood monocyte-derived macrophages and identified the M. tuberculosis gene corresponding the mutated M. smegmatis gene for five of them.
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