Words similar to monocyte-derived
Example sentences for: monocyte-derived
How can you use “monocyte-derived” in a sentence? Here are some example sentences to help you improve your vocabulary:
In the studies reported here, we used a similar enrichment scheme to identify two additional recombinant M. smegmatis clones, sur2 and sur3, that demonstrated enhanced survival during infections of THP-1 human monocyte-derived macrophages.
To enrich for clones with increased survival in human macrophages, the library was passed through THP-1 macrophages as shown schematically in Figure 1. THP-1 is a human monocyte-derived macrophage cell line [ 21 ] and wild-type M. smegmatis bacteria are rapidly killed by THP-1 macrophages [ 22 ] . After three rounds of enrichment, the resulting clones were analyzed individually by Southern blot to evaluate the number of different clones present as previously described [ 20 ] . Out of the 3000 colonies recovered, genomic DNAs from the bacteria of 90 randomly chosen colonies were digested with Pst I and hybridized with the pYUB178 vector.
MDC, monocyte-derived cells; b, basal layer; pb, parabasal layers; im, intermediate layers; s, superficial layers; Ip, lamina propria; MHC, major histocompatibility complex; Ig, immunoglobulin; DC, dendritic cells; TCU, tissue control unit; PBS, phosphate-buffered saline; FITC, fluorescein-isothiocyanate; IHC, immunohistochemistry.
Genes identified using enrichment or screening protocols include Rv2962c and Rv2958c (probable glucuronsyl transferases), Rv2220 (glutamine synthetase A1), Rv3913-Rv3914 (thioredoxin, thioredoxin reductase) and Rv2416c ( eis, unknown function) [ 14 15 22 29 ] . By screening insertional mutants of M. smegmatis, Lagier et al [ 30 ] isolated 8 mutants with impaired ability to survive in human peripheral blood monocyte-derived macrophages and identified the M. tuberculosis gene corresponding the mutated M. smegmatis gene for five of them.
Interestingly, although lamina propria of ectocervix showed the presence of primitive (CD14) moderately (HLA-DR) and well differentiated MDC (CD68), the monocyte-derived cells entering epithelium appear to initiate a new differentiation associated with CD1a expression and HLA-DR and CD68 secretion.
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