Words similar to momlv
Example sentences for: momlv
How can you use “momlv” in a sentence? Here are some example sentences to help you improve your vocabulary:
cfu = colony forming units; COX-2 = cyclooxygenase-2; DMEM = Dulbecco's modified Eagle's medium; EGFP = enhanced green fluorescent protein; FACS = fluorescence-activated cell sorting; FLS = fibroblast-like synovial cells; MoMLV = Moloney murine leukemia virus; PCR = polymerase chain reaction; RA = rheumatoid arthritis; RCF = relative centrifugal force.
A similar, modified MoMLV vector has been used to stably express EGFP in human marrow stromal cells [ 15 ] , another fibroblast-like primary cell type.
First, our viral backbone is a modified MoMLV vector that incorporates genetic elements (myeloproliferative sarcoma virus long-terminal repeats and B2 mutation) associated with resistance to transcriptional silencing following proviral integration in primary cells [ 7 8 ] . While we did not perform a detailed comparison of EGFP expression in FLS using the modified and unmodified vector backbones, preliminary experiments suggested that the modified vector was superior (J Yang, unpublished observations).
In these experiments, we used a modified MoMLV vector (pRET2), designed to improve transcriptional stability in primary cells.
Previous studies exploring the use of recombinant MoMLV vectors concluded that FLS were relatively resistant to transduction [ 2 3 4 5 ] , limiting enthusiasm for this approach.