Words similar to moloney
Example sentences for: moloney
How can you use “moloney” in a sentence? Here are some example sentences to help you improve your vocabulary:
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
Synovial tissues isolated from patients with rheumatoid arthritis (RA) display biologic properties that differ from 'normal' synovium, and there is a rapidly expanding catalogue of biochemical and molecular changes that underlie this phenotype [ 1 ] . We have investigated the feasibility of using Moloney murine leukemia virus (MoMLV) based vectors to constitutively express cloned genes in primary human fibroblast-like synovial cells (FLS), with the long-term objective of defining the contributions of specific signaling pathways and inflammatory mediators to the destructive phenotype of FLS in RA.
We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] . Here, we compare variation in Tag levels in 3 of these lines and 3 additional lines (termed tkT lines), immortalized with Moloney Sarcoma Virus vectors expressing Tag from an internal herpes thymidine kinase (tk) promoter.
Gene trap retroviruses developed in our laboratory contain a selectable marker in the U3 region of the long terminal repeat (LTR) of a replication-defective Moloney murine leukemia virus.
cfu = colony forming units; COX-2 = cyclooxygenase-2; DMEM = Dulbecco's modified Eagle's medium; EGFP = enhanced green fluorescent protein; FACS = fluorescence-activated cell sorting; FLS = fibroblast-like synovial cells; MoMLV = Moloney murine leukemia virus; PCR = polymerase chain reaction; RA = rheumatoid arthritis; RCF = relative centrifugal force.
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