Words similar to moloney
Example sentences for: moloney
How can you use “moloney” in a sentence? Here are some example sentences to help you improve your vocabulary:
For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).
Briefly, RNA was treated with DNAse I and then 2 μg was reverse transcribed with Moloney murine leukemia virus reverse transcriptase and oligodT primers.
However, the sequence of the PCR product was identical to the wild-type Moloney murine leukemia virus LTR (data not shown).
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
Gene trap retroviruses developed in our laboratory contain a selectable marker in the U3 region of the long terminal repeat (LTR) of a replication-defective Moloney murine leukemia virus.
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