Words similar to moloney
Example sentences for: moloney
How can you use “moloney” in a sentence? Here are some example sentences to help you improve your vocabulary:
Briefly, RNA was treated with DNAse I and then 2 μg was reverse transcribed with Moloney murine leukemia virus reverse transcriptase and oligodT primers.
For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).
However, the sequence of the PCR product was identical to the wild-type Moloney murine leukemia virus LTR (data not shown).
We have previously characterized clonal mouse astrocyte cell lines, immortalized by transduction of SV40 large T antigen expressed from the MoMuLV LTR [ 6 13 ] . Here, we compare variation in Tag levels in 3 of these lines and 3 additional lines (termed tkT lines), immortalized with Moloney Sarcoma Virus vectors expressing Tag from an internal herpes thymidine kinase (tk) promoter.
Synovial tissues isolated from patients with rheumatoid arthritis (RA) display biologic properties that differ from 'normal' synovium, and there is a rapidly expanding catalogue of biochemical and molecular changes that underlie this phenotype [ 1 ] . We have investigated the feasibility of using Moloney murine leukemia virus (MoMLV) based vectors to constitutively express cloned genes in primary human fibroblast-like synovial cells (FLS), with the long-term objective of defining the contributions of specific signaling pathways and inflammatory mediators to the destructive phenotype of FLS in RA.
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