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Example sentences for: molegos
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These results indicate that molegos can provide a useful basis for identifying specificity determining regions in enzymes with similar active sites but different activity spectra [ 46 28 3 ] . Site directed mutagenesis based on these results can define the function of the unique elements of the APEs, and aid in the design of enzymes with altered specificity.
NO analyzed the structural properties of the molegos, and prepared figures 2, 3, 4, 5, 6, 7. TI prepared mutants of human APE1 protein to test the conclusions of this paper, and he provided background and list of mutations affecting APE function.
The extensive contacts that APE1 makes to these positions are mediated by molegos it does not share with DNase 1. Molegos 6, 7a and 10 all have residues within hydrogen bonding distance of the three basepairs 3' of the AP-site.
The 12 conserved molegos form the β-barrel core of huAPE1.
Thus, molegos can effectively guide the redesign of enzymes to alter specificity.