Words similar to moesin

Example sentences for: moesin

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These include SH3 domains (Src homology 3 domains, that bind to target proteins), MYTH4 (a domain of unknown function found in a few classes of myosins), a zinc-binding domain, a pleckstrin homology domain, FERM/talin (band 4.1/ezrin/radixin/moesin), GPA-rich domains and a protein kinase domain [ 8, 22, 26].
Crosslinking of polypeptides was also evaluated after treatment with several other fixatives that preserved microextensions well and that were found to be useful previously for moesin staining (Figure 6B).
Affinity-purified polyclonal antibodies (95/1) were used for the identification of moesin by immunoblotting and for immunofluorescence.
Although extraction can be done before, during, or after fixation, we used simultaneous extraction and fixation, because (i) cytoskeletal molecules may be rearranged or altered during fixation, as irreversible crosslinking of polypeptides is not complete even after 15 min incubation with 4% PFA at 37°C; (ii) although actin was more difficult to extract, moesin and other polypeptides were removed already with 0.5% DOTMAC in the absence of PFA.
One possibility is that moesin in NIH3T3 cells is modified differently.