Words similar to moesin
Example sentences for: moesin
How can you use “moesin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although extraction can be done before, during, or after fixation, we used simultaneous extraction and fixation, because (i) cytoskeletal molecules may be rearranged or altered during fixation, as irreversible crosslinking of polypeptides is not complete even after 15 min incubation with 4% PFA at 37°C; (ii) although actin was more difficult to extract, moesin and other polypeptides were removed already with 0.5% DOTMAC in the absence of PFA.
We have previously shown that purified threonine558-phosphomoesin selectively co-sedimented with actin filaments in the presence of DOTMAC or PIPs, but excess Triton X-100 disrupted the interaction of moesin with PIPs [ 19].
DOTMAC/PFA was superior to TCA and the standard Triton X-100 permeabilization procedure to preserve microextensions and for moesin staining (Figure 4, G, K, and 4N,O,P).
These include SH3 domains (Src homology 3 domains, that bind to target proteins), MYTH4 (a domain of unknown function found in a few classes of myosins), a zinc-binding domain, a pleckstrin homology domain, FERM/talin (band 4.1/ezrin/radixin/moesin), GPA-rich domains and a protein kinase domain [ 8, 22, 26].
These results were consistent with a model of an allosteric change induced by PIPs (or DOTMAC) that exposed and maintained a cytoskeleton-binding site of moesin.
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