Words similar to mlh
Example sentences for: mlh
How can you use “mlh” in a sentence? Here are some example sentences to help you improve your vocabulary:
The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
We examined the status of promoter hypermethylation of 16 genes ( CDH1 , DAPK , RARB , HIC1 , FHIT , RASSF1A , APC , CDKN2A , MGMT , BRCA1 , TP73 , TIMP3 , GSTP1 , MLH1 , p14 ARF , and RB1 ) in eight specimens of normal cervical squamous epithelia and 90 CC specimens.
Aberrant promoter methylation changes that occur in cancer are associated with transcriptional repression and loss of function of the gene by interrupting the binding of proteins involved in transcription activator complex [ 12 ] . Our gene expression analysis by RT-PCR demonstrated that all tumors that showed methylation of MGMT and MLH1 also showed down-regulated expression, while RASSF1A and RARB genes showed down-regulation of mRNA levels in most of the methylated tumors.
Levels of expression of each gene were assessed by comparing with the respective control values, obtained from the averages calculated from 2 to 4 normal testes, after normalization against ACTB . All tumors with promoter hypermethylation of the MGMT and MLH1 genes exhibited an absence or down-regulated expression of the respective gene, while 8 of 10 cases with RASSF1A methylation and 3 of 5 tumors with RARB methylation showed down-regulated expression (Table 1).
To examine the biological role of promoter methylation in CC, we assessed the levels of mRNA by semi-quantitative RT-PCR in eight cell lines and compared with the respective control values obtained from the averages calculated from 2 normal cervix after normalization against ACTB . This analysis was performed on 7 genes ( CDH1 , DAPK , RARB , HIC1 , MGMT , RASSF1A , and MLH1 ) that exhibited methylation in one or more cell lines and primary tumors (Fig.
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