Words similar to mislocalized
Example sentences for: mislocalized
How can you use “mislocalized” in a sentence? Here are some example sentences to help you improve your vocabulary:
The resulting enzyme is both mislocalized and unstable [ 27 ] . Another mutation, Q1098P, was found to cause retention of SI in the transport pathway between the endoplasmic reticulum and Golgi apparatus [ 28 ] . An unusual mutation, Q117R, that alters the structure close to the O-glycosylated stalk domain, leads to random distribution of the enzyme to both the apical and basolateral cell membranes [ 29 ] . An unusual human mutation that causes SI to be secreted rather than retained in the plasma membrane demonstrates that the enzyme is required to be at the enterocyte cell surface [ 30 ] .
To determine if additional, non-GFP tagged Nups were mislocalized in these cells, we used indirect immunofluorescence (IF) microscopy with antibodies directed against either Nup116p or Pom152p.
Although prlA and prlG strains export secretory proteins that completely lack a signal sequence, there is no evidence of export of any cytoplasmic proteins in a prl suppressor strain [ 13 ] . That is, proteins that are supposed to remain in the cytoplasm are not mislocalized to the periplasm.
Logarithmically growing cultures of npa1-1/sec23 and npa2-1/sec13 were shifted to the non-permissive temperature as before and then fixed and processed for IF with polyclonal antibodies against Kar2p to visualize the ER [ 45 ] . The monoclonal antibody mAb414, which recognizes a subset of Nups distinct from Nic96p and Nup170p [ 46 ] , was also used because the GFP fluorescence of the GFP-Nups was more sensitive to fixation when the GFP-Nups were mislocalized than when they remained at the nuclear rim (unpublished observation).
The lack of secretion defects in some of the mutants indicates that non-nucleoporins are being trafficked properly when Nups are mislocalized.
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